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anti-FOXA3 antibody :: Rabbit FOXA3 Polyclonal Antibody

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Catalog # MBS462181
Unit / Price
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  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

FOXA3, Polyclonal Antibody

Full Product Name

Rabbit anti FOXA3/HNF3G Polyclonal antibody

Product Synonym Names
Rabbit anti-FOXA3; Forkhead Box A2 (FOXA3); Hepatocyte nuclear factor 3 gamma (HNF3G)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (46367518..46377055). Location: 19q13.2-q13.4
OMIM
602295
3D Structure
ModBase 3D Structure for P55318
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Specificity
This antibody recognizes ~37 kDa of FOXA3 protein. It also reacts with human, mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity qPurification.
Form/Format
This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the C-terminus of human FOXA3 protein. This sequence is identical to rat, mouse and human origins.
Positive Control
Huh 7 cells
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-FOXA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FOXA3 antibody
The forkhead box gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA1 plays an important role in breast cancer. The FOXA subfamily is composed of the transcription factors FOXA1, A2, A3. They were discovered as the regulators of hepatic genes. Foxa1 has been shown to be required for glucagon secretion in the pancreas, whereas Foxa2 is critical for the regulation of insulin secretion in pancreatic beta-cells. Foxa3 is important in the maintenance of glucose homeostasis, it is the dominating transcriptional regulator of GLUT2 expression in hepatocytes in vivo. In the normal activation of HNF-4alpha, HNF-1alpha, and PGC-1 induced by fasting is attenuated in mice lacking Foxa3.
Applications Tested/Suitable for anti-FOXA3 antibody
ELISA, Western Blot
Application Notes for anti-FOXA3 antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml

Testing Data of anti-FOXA3 antibody
anti-FOXA3 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for FOXA3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~37 kDa
NCBI Official Full Name
forkhead box A3
NCBI Official Synonym Full Names
forkhead box A3
NCBI Official Symbol
FOXA3  [Similar Products]
NCBI Official Synonym Symbols
FKHH3; HNF3G; TCF3G; MGC10179
  [Similar Products]
NCBI Protein Information
hepatocyte nuclear factor 3-gamma; HNF-3G; TCF-3G; HNF-3-gamma; forkhead box protein A3; transcription factor 3G; fork head-related protein FKH H3; hepatocyte nuclear factor 3, gamma
UniProt Protein Name
Hepatocyte nuclear factor 3-gamma
UniProt Synonym Protein Names
Fork head-related protein FKH H3; Forkhead box protein A3; Transcription factor 3G
Protein Family
UniProt Gene Name
FOXA3  [Similar Products]
UniProt Synonym Gene Names
HNF3G; TCF3G  [Similar Products]
UniProt Entry Name
FOXA3_HUMAN
NCBI Summary for FOXA3
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved. [provided by RefSeq]
UniProt Comments for FOXA3
Function: Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites

By similarity. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis. Ref.9

Subunit structure: Interacts with FOXA2. Ref.10

Subcellular location: Nucleus Ref.11.

Tissue specificity: Expressed in erythroleukemia and hepatoma cell lines and in liver and pancreas. Not expressed in any other cell lines or tissues examined. Ref.1

Developmental stage: Detected in prenatal liver nuclear extracts (12.4-27 weeks estimated gestational age). Not detected in postnatal liver samples. Ref.11

Sequence similarities: Contains 1 fork-head DNA-binding domain.
Product References and Citations for anti-FOXA3 antibody
Shen W et al. Foxa3 (hepatocyte nuclear factor 3gamma) is required for the regulation of hepatic GLUT2 expression and the maintenance of glucose homeostasis during a prolonged fast. 2001 Nov 16;276 (46):42812-7.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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