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anti-FZD9 antibody :: Goat FZD9 Polyclonal Antibody

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Catalog # MBS420499
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

FZD9, Polyclonal Antibody

Full Product Name

Goat anti-FZD9 Antibody

Product Synonym Names
FZD9; frizzled homolog 9 (Drosophila); FZD3; frizzled 9; FZD9 antibody; frizzled homolog 9 (Drosophila) antibody; FZD3 antibody; frizzled 9 antibody
Antibody/Peptide Pairs
FZD9 peptide (MBS426060) is used for blocking the activity of FZD9 antibody (MBS420499)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
TKTDPSLENP TH
OMIM
601766
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-TKTDPSLENPTH, from the C Terminus of the protein sequence according to NP_003499.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-FZD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FZD9 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-FZD9 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Testis shows acrosomal staining of spermatids. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments in Kidney lysates from Human, Mouse and Rat gave no specific signal but low background (at antibody concentration up to 1ug/ml).

Immunohistochemistry (IHC) of anti-FZD9 antibody
(5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-FZD9 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for FZD9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
64,466 Da
NCBI Official Full Name
frizzled-9
NCBI Official Synonym Full Names
frizzled class receptor 9
NCBI Official Symbol
NCBI Official Synonym Symbols
FZD3; CD349
  [Similar Products]
NCBI Protein Information
frizzled-9
UniProt Protein Name
Frizzled-9
UniProt Synonym Protein Names
FzE6; CD_antigen: CD349
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FZD3; Fz-9; hFz9  [Similar Products]
UniProt Entry Name
FZD9_HUMAN
NCBI Summary for FZD9
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]
UniProt Comments for FZD9
FZD9: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, multi-pass; GPCR, Fz/Smo family; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cell surface; cytoplasm; filopodium membrane; integral to membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein heterodimerization activity; protein homodimerization activity; Wnt receptor activity; Wnt-protein binding

Biological Process: B cell differentiation; G-protein coupled receptor protein signaling pathway; learning and/or memory; nervous system development; neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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