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anti-GAD2 antibody :: Rabbit GAD65 Polyclonal Antibody

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Catalog # MBS175471
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

GAD65 (GAD2), Polyclonal Antibody

Full Product Name

Anti-GAD65 antibody

Product Synonym Names
Glutamate decarboxylase 2; glutamate decarboxylase 2(pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase antibody; DCE 2 antibody; DCE2 antibody; DCE2_HUMAN antibody; GAD 2 antibody; GAD 65 antibody; GAD-2 antibody; GAD-65 antibody; Gad2 antibody; Glutamate decarboxylase 2(pancreas) antibody; Glutamate Decarboxylase 2(pancreatic islets and brain 65kDa) antibody; Glutamate decarboxylase 2 antibody; Glutamate Decarboxylase 65 antibody; Glutamate decarboxylase 65 kDa isoform antibody; Glutamic Acid Decarboxylase 2 antibody; Glutamic Acid Decarboxylase 65 antibody; MGC161605 antibody; MGC161607 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
138275
3D Structure
ModBase 3D Structure for Q05329
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65 (21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GAD2 antibody
Description: Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Glutamate decarboxylase or glutamic acid decarboxylase(GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD65 is the isoform with molecular weight of 65 kDa. Karlsen et al.(1991) mapped the GAD2 gene to chromosome 10p13-p11.2 by in situ hybridization. Bu et al.(1992) isolated a cDNA corresponding to the GAD2 gene from a human brain cDNA library. The deduced 585-amino acid protein has a molecular mass of 65 kD. GAD65 is target of autoantibodies in people who later develop type 1 diabetes mellitus or latent autoimmune diabetes.
Applications Tested/Suitable for anti-GAD2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB) of anti-GAD2 antibody
Anti-GAD65 antibody, MBS175471, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate

anti-GAD2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GAD2 antibody
Anti-GAD65 antibody, MBS175471, IHC(P)
IHC(P): Rat Brain Tissue
anti-GAD2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,411 Da
NCBI Official Full Name
Glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
NCBI Official Symbol
NCBI Official Synonym Symbols
GAD65
  [Similar Products]
NCBI Protein Information
glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Synonym Protein Names
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GAD65; GAD-65  [Similar Products]
UniProt Entry Name
DCE2_HUMAN
NCBI Summary for GAD2
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
UniProt Comments for GAD2
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 4.1.1.15; Carbohydrate Metabolism - butanoate; Lyase; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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