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anti-GADD45A antibody :: Rabbit GADD45 alpha Polyclonal Antibody

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Catalog # MBS8247390
Unit / Price
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  0.05 mL  /  $195 +1 FREE 8GB USB
  0.1 mL  /  $250 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

GADD45 alpha (GADD45A), Polyclonal Antibody

Popular Item
Full Product Name

Anti-GADD45 alpha Antibody

Product Synonym Names
DDIT1; GADD45; Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
126335
3D Structure
ModBase 3D Structure for P24522
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of GADD45 alpha protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
Recombinant full length protein of human GADD45 alpha
Preparation and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-GADD45A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GADD45A antibody
Rabbit polyclonal antibody to GADD45 alpha
Applications Tested/Suitable for anti-GADD45A antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-GADD45A antibody
WB: 1/500 - 1/2000
IF/IC: 1/10 - 1/100

Western Blot (WB) of anti-GADD45A antibody
Western blot analysis of GADD45 alpha expression in mouse liver (A), mouse kidney (B) whole cell lysates.
anti-GADD45A antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-GADD45A antibody
Immunofluorescent analysis of GADD45 alpha staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
anti-GADD45A antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for GADD45A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
growth arrest and DNA damage-inducible protein GADD45 alpha isoform 2
NCBI Official Synonym Full Names
growth arrest and DNA damage inducible alpha
NCBI Official Symbol
GADD45A  [Similar Products]
NCBI Official Synonym Symbols
DDIT1; GADD45
  [Similar Products]
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Synonym Protein Names
DNA damage-inducible transcript 1 protein; DDIT-1
UniProt Gene Name
GADD45A  [Similar Products]
UniProt Synonym Gene Names
DDIT1; GADD45; DDIT-1  [Similar Products]
UniProt Entry Name
GA45A_HUMAN
NCBI Summary for GADD45A
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]
UniProt Comments for GADD45A
GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Cell cycle regulation; Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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