• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-GARS antibody :: Rabbit GARS Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS7048225
Unit / Price
Scan QR to view Datasheet
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

GARS, Polyclonal Antibody

Popular Item
Full Product Name

GARS Antibody

Product Synonym Names
Glycine--tRNA ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS; GARS
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS7048225 Technical Datasheet
OMIM
D30658 mRNA
3D Structure
ModBase 3D Structure for P41250
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Glycine--tRNA ligase protein (1-389AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-69485 / sc-69486 / sc-98614
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-GARS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GARS antibody
Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Applications Tested/Suitable for anti-GARS antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-GARS antibody
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
IP: 1: 200-1: 2000

Western Blot (WB) of anti-GARS antibody
Western Blot
Positive WB detected in: THP-1 whole cell lysate,U87 whole cell lysate,HL-60 whole cell lysate,Mouse brain tissue,Mouse liver tissue,Rat brain tissue,Rat liver tissue
All lanes: GARS antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 KDa
Observed band size: 84,50 KDa

anti-GARS antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GARS antibody
Immunohistochemistry of paraffin-embedded human testis tissue using MBS7048225 at dilution of 1:100
anti-GARS antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-GARS antibody
Immunofluorescent analysis of HepG2 cells using MBS7048225 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-GARS antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-GARS antibody
Immunoprecipitating GARS in HL-60 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1ug)instead of MBS7048225 in HL-60 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS7048225 (8ug)+ HL-60 whole cell lysate (500ug)
Lane 3: HL-60 whole cell lysate (20ug)

anti-GARS antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for GARS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
83,166 Da
NCBI Official Full Name
glycine--tRNA ligase isoform 2
NCBI Official Synonym Full Names
glycyl-tRNA synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
HMN5; CMT2D; DSMAV; GlyRS; SMAD1
  [Similar Products]
NCBI Protein Information
glycine--tRNA ligase
UniProt Protein Name
Glycine--tRNA ligase
UniProt Synonym Protein Names
Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AP-4-A synthetase; GlyRS  [Similar Products]
UniProt Entry Name
SYG_HUMAN
NCBI Summary for GARS
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
UniProt Comments for GARS
GARS: Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Defects in GARS are the cause of Charcot-Marie-Tooth disease type 2D (CMT2D). CMT2D is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2D is characterized by a more severe phenotype in the upper extremities (severe weakness and atrophy, absence of tendon reflexes) than in the lower limbs. CMT2D inheritance is autosomal dominant. Defects in GARS are a cause of distal hereditary motor neuronopathy type 5A (HMN5A); also known as distal hereditary motor neuropathy type V (DSMAV). A disorder characterized by distal muscular atrophy mainly affecting the upper extremities, in contrast to other distal motor neuronopathies. These constitute a heterogeneous group of neuromuscular diseases caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.14; Ligase; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: axon; cytoplasm; cytosol; mitochondrial matrix; nucleoplasm

Molecular Function: bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; glycine-tRNA ligase activity; protein dimerization activity

Biological Process: diadenosine tetraphosphate biosynthetic process; glycyl-tRNA aminoacylation; tRNA aminoacylation for protein translation

Disease: Charcot-marie-tooth Disease, Axonal, Type 2d; Neuronopathy, Distal Hereditary Motor, Type Va
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-GARS antibody
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000