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anti-GPAM antibody :: Rabbit GPAT1 Polyclonal Antibody

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Catalog # MBS150163
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

GPAT1 (GPAM), Polyclonal Antibody

Full Product Name

GPAT1 Antibody

Product Synonym Names
GPAT1; GPAT; GPAT1; KIAA1560; Glycerol-3-phosphate acyltransferase 1; mitochondrial; GPAT-1; glycerol-3-phosphate acyltransferase, mitochondrial
Antibody/Peptide Pairs
GPAT1 peptide (MBS152752) is used for blocking the activity of GPAT1 antibody (MBS150163)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602395
3D Structure
ModBase 3D Structure for Q9HCL2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
GPAT1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1.
Buffer
GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
GPAT1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-GPAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GPAM antibody
GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.
Applications Tested/Suitable for anti-GPAM antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GPAM antibody
GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-GPAM antibody
Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.
anti-GPAM antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GPAM antibody
Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.
anti-GPAM antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for GPAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
93,795 Da
NCBI Official Full Name
glycerol-3-phosphate acyltransferase 1, mitochondrial
NCBI Official Synonym Full Names
glycerol-3-phosphate acyltransferase, mitochondrial
NCBI Official Symbol
NCBI Official Synonym Symbols
GPAT; GPAT1
  [Similar Products]
NCBI Protein Information
glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT-1; glycerol 3-phosphate acyltransferase, mitochondrial
UniProt Protein Name
Glycerol-3-phosphate acyltransferase 1, mitochondrial
UniProt Gene Name
UniProt Synonym Gene Names
GPAT1; KIAA1560; GPAT-1  [Similar Products]
UniProt Entry Name
GPAT1_HUMAN
NCBI Summary for GPAM
This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for GPAM
GPAM: glycerol-3-phosphate acyltransferase is an integral multi-pass enzyme of the mitochondrial outer membrane. It performs the first step in de novo phospholipid biosynthesis. Member of the phospholipid and glycerol acyltransferase family

Protein type: Lipid Metabolism - glycerolipid; Mitochondrial; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Transferase; Membrane protein, integral; EC 2.3.1.15

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: mitochondrial outer membrane; mitochondrial inner membrane; integral to membrane; plasma membrane

Molecular Function: glycerol-3-phosphate O-acyltransferase activity

Biological Process: regulation of cytokine secretion; acyl-CoA metabolic process; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; positive regulation of multicellular organism growth; fatty acid metabolic process; positive regulation of activated T cell proliferation; fatty acid homeostasis; phospholipid metabolic process; response to glucose stimulus; phosphatidic acid biosynthetic process; CDP-diacylglycerol biosynthetic process; defense response to virus; phospholipid homeostasis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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