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anti-GRB10 antibody :: Rabbit GRB10 Polyclonal Antibody

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Catalog # MBS150671
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

GRB10, Polyclonal Antibody

Also Known As

GRB10 Antibody

Product Synonym Names
GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10; GRBIR; KIAA0207; Growth factor receptor-bound protein 10; GRB10 adapter protein; growth factor receptor-bound protein 10
Antibody/Peptide Pairs
GRB10 peptide (MBS152893) is used for blocking the activity of GRB10 antibody (MBS150671)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
594
OMIM
601523
3D Structure
ModBase 3D Structure for Q13322
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.
Purity/Purification
GRB10 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10.
Buffer
GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
GRB10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-GRB10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRB10 antibody
GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.
Applications Tested/Suitable for anti-GRB10 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-GRB10 antibody
GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-GRB10 antibody
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.
anti-GRB10 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-GRB10 antibody
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.
anti-GRB10 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for GRB10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
66,487 Da
NCBI Official Full Name
growth factor receptor-bound protein 10 isoform a
NCBI Official Synonym Full Names
growth factor receptor-bound protein 10
NCBI Official Symbol
GRB10  [Similar Products]
NCBI Official Synonym Symbols
RSS; IRBP; MEG1; GRB-IR; Grb-10
  [Similar Products]
NCBI Protein Information
growth factor receptor-bound protein 10; GRB10 adapter protein; GRB10 adaptor protein; maternally expressed gene 1; insulin receptor-binding protein Grb-IR
UniProt Protein Name
Growth factor receptor-bound protein 10
UniProt Synonym Protein Names
GRB10 adapter protein; Insulin receptor-binding protein Grb-IR
UniProt Gene Name
GRB10  [Similar Products]
UniProt Synonym Gene Names
GRBIR; KIAA0207  [Similar Products]
UniProt Entry Name
GRB10_HUMAN
NCBI Summary for GRB10
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]
UniProt Comments for GRB10
GRB10: an adaptor protein that inhibits insulin-stimulated IRS-PI (3)K-Akt signaling pathway by disrupting the association of IRS with the insulin receptor. Also binds activated PDGFR and EGFR. Preferentially localizes to the mitochondria, modulating the anti-apoptotic activity of mitochondrial Raf-1. Can translocate to the plasma membrane and lamellipodia. Three alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; insulin receptor binding

Biological Process: negative regulation of glucose import; negative regulation of Wnt receptor signaling pathway; insulin-like growth factor receptor signaling pathway; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphorylation; response to insulin stimulus; negative regulation of glycogen biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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