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anti-HADH antibody :: Rabbit HADH Polyclonal Antibody

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Catalog # MBS126736
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

HADH, Polyclonal Antibody

Popular Item
Full Product Name

HADH Polyclonal Antibody

Product Synonym Names
HADH; HAD; HADH1; HADHSC; HHF4; M/SCHAD; MGC8392; SCHAD
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 609975
3D Structure
ModBase 3D Structure for Q16836
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human HADH
Calculated Molecular Weight
34kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-HADH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HADH antibody
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
Product Categories/Family for anti-HADH antibody
Applications Tested/Suitable for anti-HADH antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-HADH antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-HADH antibody
Western blot analysis of extracts of various cell lines, using HADH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
anti-HADH antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HADH antibody
Immunohistochemistry of paraffin-embedded rat liver using HADH antibody at dilution of 1:100 (40x lens).
anti-HADH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HADH antibody
Immunohistochemistry of paraffin-embedded human liver cancer using HADH antibody at dilution of 1:100 (40x lens).
anti-HADH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HADH antibody
Immunohistochemistry of paraffin-embedded mouse liver using HADH antibody at dilution of 1:100 (40x lens).
anti-HADH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HADH antibody
Immunohistochemistry of paraffin-embedded rat spinal cord using HADH antibody at dilution of 1:100 (40x lens).
anti-HADH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HADH antibody
Immunohistochemistry of paraffin-embedded human tonsil using HADH antibody at dilution of 1:100 (40x lens).
anti-HADH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HADH antibody
Immunohistochemistry of paraffin-embedded mouse brain using HADH antibody at dilution of 1:100 (40x lens).
anti-HADH antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HADH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
314
NCBI Official Full Name
Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
hydroxyacyl-CoA dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD
  [Similar Products]
NCBI Protein Information
hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; short-chain 3-hydroxyacyl-CoA dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase
UniProt Protein Name
Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase
UniProt Gene Name
UniProt Synonym Gene Names
HAD; HADHSC; SCHAD; HCDH  [Similar Products]
UniProt Entry Name
HCDH_HUMAN
NCBI Summary for HADH
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
UniProt Comments for HADH
HADHSC: Plays an essential role in the mitochondrial beta- oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency). HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death. Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4); also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion. Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid elongation in mitochondria; Oxidoreductase; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate; EC 1.1.1.35; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Chromosomal Location of Human Ortholog: 4q22-q26

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane

Molecular Function: 3-hydroxyacyl-CoA dehydrogenase activity

Biological Process: response to drug; fatty acid beta-oxidation; cellular lipid metabolic process; response to activity; response to insulin stimulus; negative regulation of insulin secretion

Disease: 3-hydroxyacyl-coa Dehydrogenase Deficiency; Hyperinsulinemic Hypoglycemia, Familial, 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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