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anti-TSC1 antibody :: Rabbit Hamartin Polyclonal Antibody

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Catalog # MBS176028
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

Hamartin (TSC1), Polyclonal Antibody

Popular Item
Also Known As

Anti-Hamartin antibody

Product Synonym Names
Hamartin; tuberous sclerosis 1; Hamartin antibody; kiaa0243 antibody; LAM antibody; TSC antibody; TSC1 antibody; Tsc1 gene antibody; TSC1_HUMAN antibody; Tuberous sclerosis 1 antibody; Tuberous sclerosis 1 protein antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1164
OMIM
gene 607341
3D Structure
ModBase 3D Structure for Q92574
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin (1150-1164aa QLHIMDYNETHHEHS), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-TSC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TSC1 antibody
Description: Rabbit IgG polyclonal antibody for Hamartin(TSC1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: TSC1(Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al.(1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al.(2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al.(1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.
Applications Tested/Suitable for anti-TSC1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-TSC1 antibody
Western Blot (WB):
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: n/a

Immunohistrochemistry (IHC) - Paraffin-embedded Section: Concentration: 0.5-1ug/ml
Tested Species: Hu
Predicted Species: Ms,Rat
Antigen Retrieval: By Heat

WB: the detection limit for TSC1 is approximately 5ng/lane under reducing conditions.
Tested Species: in-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of fomalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-TSC1 antibody
Anti-Hamartin antibody, MBS176028, Western blotting
All lanes: Anti Hamartin (MBS176028) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 160KD
anti-TSC1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TSC1 antibody
Anti-Hamartin antibody, MBS176028, IHC(P)
IHC(P): Human Mammary Cancer Tissue
anti-TSC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-TSC1 antibody
Anti-Hamartin antibody, MBS176028, IHC(P)
IHC(P): Rat Brain Tissue
anti-TSC1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TSC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
124,015 Da
NCBI Official Full Name
Hamartin
NCBI Official Synonym Full Names
tuberous sclerosis 1
NCBI Official Symbol
NCBI Official Synonym Symbols
LAM; TSC
  [Similar Products]
NCBI Protein Information
hamartin; tumor suppressor; tuberous sclerosis 1 protein
UniProt Protein Name
Hamartin
UniProt Synonym Protein Names
Tuberous sclerosis 1 protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0243; TSC  [Similar Products]
UniProt Entry Name
TSC1_HUMAN
NCBI Summary for TSC1
This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
UniProt Comments for TSC1
TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; protein complex; growth cone; intracellular membrane-bound organelle; membrane; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; protein N-terminus binding; GTPase activating protein binding; GTPase regulator activity

Biological Process: myelination; protein heterooligomerization; regulation of cell-matrix adhesion; regulation of cell cycle; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; cell projection organization and biogenesis; positive regulation of focal adhesion formation; synapse organization and biogenesis; kidney development; cell cycle arrest; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; negative regulation of translation; regulation of stress fiber formation; glucose import; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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