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anti-HIST1H3D antibody :: Rabbit HIST1H3D Polyclonal Antibody

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Catalog # MBS9126667
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

HIST1H3D, Polyclonal Antibody

Popular Item
Full Product Name

HIST1H3D Polyclonal Antibody

Product Synonym Names
H3/b; H3FB
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 602819
3D Structure
ModBase 3D Structure for P68431
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human HIST1H3D
Calculated Molecular Weight
15kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-HIST1H3D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HIST1H3D antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Product Categories/Family for anti-HIST1H3D antibody
Applications Tested/Suitable for anti-HIST1H3D antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-HIST1H3D antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100

Western Blot (WB) of anti-HIST1H3D antibody
Western blot analysis of extracts of various cell lines, using HIST1H3D antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
anti-HIST1H3D antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HIST1H3D antibody
Immunohistochemistry of paraffin-embedded rat spleen using HIST1H3D antibody at dilution of 1:100 (40x lens).
anti-HIST1H3D antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HIST1H3D antibody
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIST1H3D antibody at dilution of 1:100 (40x lens).
anti-HIST1H3D antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HIST1H3D antibody
Immunohistochemistry of paraffin-embedded human kidney using HIST1H3D antibody at dilution of 1:100 (40x lens).
anti-HIST1H3D antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HIST1H3D antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using HIST1H3D antibody at dilution of 1:100 (40x lens).
anti-HIST1H3D antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HIST1H3D antibody
Immunohistochemistry of paraffin-embedded human stomach using HIST1H3D antibody at dilution of 1:100 (40x lens).
anti-HIST1H3D antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HIST1H3D antibody
Immunohistochemistry of paraffin-embedded mouse cancer using HIST1H3D antibody at dilution of 1:100 (40x lens).
anti-HIST1H3D antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-HIST1H3D antibody
Immunofluorescence analysis of U2OS cells using HIST1H3D antibody. Blue: DAPI for nuclear staining.
anti-HIST1H3D antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for HIST1H3D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
136
NCBI Official Full Name
Histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3j
NCBI Official Symbol
HIST1H3J  [Similar Products]
NCBI Official Synonym Symbols
H3/j; H3FJ
  [Similar Products]
NCBI Protein Information
histone H3.1; histone H3/j; histone 1, H3j; H3 histone family, member J
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name
HIST1H3A  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
H31_HUMAN
NCBI Summary for HIST1H3D
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Comments for HIST1H3D
H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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