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anti-HSD17B1 antibody :: Rabbit HSD17B1 Polyclonal Antibody

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Catalog # MBS177074
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

HSD17B1, Polyclonal Antibody

Popular Item
Also Known As

Anti-HSD17B1 antibody

Product Synonym Names
Estradiol 17-beta-dehydrogenase 1; hydroxysteroid(17-beta) dehydrogenase 1; 17 beta HSD 1 antibody; 17 beta hydroxysteroid dehydrogenase type 1 antibody; 17-beta-HSD 1 antibody; 17-beta-hydroxysteroid dehydrogenase type 1 antibody; 20 alpha-hydroxysteroid dehydrogenase antibody; 20-alpha-HSD antibody; DHB1_HUMAN antibody; E17KSR antibody; E2DH antibody; EDH17B1 antibody; EDH17B2 antibody; EDHB17 antibody; Estradiol 17 beta dehydrogenase 1 antibody; Estradiol 17-beta-dehydrogenase 1 antibody; HSD17 antibody; HSD17B1 antibody; Hydroxysteroid(17 beta) dehydrogenase 1 antibody; MGC13814 antibody; Placental 17 beta hydroxysteroid dehydrogenase antibody; Placental 17-beta-hydroxysteroid dehydrogenase antibody; SDR28C1 antibody; Short chain dehydrogenase/reductase family 28CE,member 1 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
328
OMIM
109684
3D Structure
ModBase 3D Structure for P14061
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, Rat
Predicted Species: Mouse
Specificity
No Cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1 (29-43aa QSFKVYATLRDLKTQ), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HSD17B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HSD17B1 antibody
Description: Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 1(HSD17B1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: HSD17B1(17-BETA-HYDROXYSTEROID DEHYDROGENASE I), also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase, is an enzyme that is responsible for the interconversion of estrone(E1) and estradiol(E2) as well as the interconversion of androstenedione and testosterone. Its cytogenetic location is 17q21.2. HSD17B1 gene expression is detected in 34 of 42(81%) adenomas in all tumor subtypes; HSD17B2 mRNA is detected in 18 of 42(43%) adenomas but not in prolactinomas. Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies. All 4 HSD17B isoforms are variably expressed in human anterior pituitary adenomas, which also show HSD17B enzyme activity, suggesting that HSD17B may play an important role in regulating the local cellular levels of estradiol.
Applications Tested/Suitable for anti-HSD17B1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes for anti-HSD17B1 antibody
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Immunocytochemistry:
Concentration: 0.5-1ug/ml
Tested Species: Hu
Predicted Species: Rat, Ms

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Immunocytochemistry (ICC) of anti-HSD17B1 antibody
Anti-HSD17B1 antibody, MBS177074, ICC
ICC: HELA Cell
anti-HSD17B1 antibody Immunocytochemistry (ICC) (ICC) image
Western Blot (WB) of anti-HSD17B1 antibody
Anti-HSD17B1 antibody, MBS177074, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: HELA Cell Lysate

anti-HSD17B1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HSD17B1 antibody
Anti-HSD17B1 antibody, MBS177074, IHC(P)
IHC(P): Rat Liver Tissue
anti-HSD17B1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HSD17B1 antibody
Anti-HSD17B1 antibody, MBS177074, IHC(P)
IHC(P): Rat Ovary Tissue
anti-HSD17B1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HSD17B1 antibody
Anti-HSD17B1 antibody, MBS177074, IHC(P)
IHC(P): Human Placenta Tissue
anti-HSD17B1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HSD17B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,950 Da
NCBI Official Full Name
Estradiol 17-beta-dehydrogenase 1
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 1
NCBI Official Symbol
HSD17B1  [Similar Products]
NCBI Official Synonym Symbols
HSD17; EDHB17; EDH17B2; SDR28C1
  [Similar Products]
NCBI Protein Information
estradiol 17-beta-dehydrogenase 1; E2DH; 20-alpha-HSD; 17-beta-HSD 1; estradiol 17-beta-dehydrogenase-1; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 1; placental 17-beta-hydroxysteroid dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 1 isoform; short chain dehydrogenase/reductase family 28CE, member 1
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 1
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 1; 17-beta-HSD 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Placental 17-beta-hydroxysteroid dehydrogenase
UniProt Gene Name
HSD17B1  [Similar Products]
UniProt Synonym Gene Names
E17KSR; EDH17B1; EDH17B2; EDHB17; 17-beta-HSD 1; 20-alpha-HSD  [Similar Products]
UniProt Entry Name
DHB1_HUMAN
UniProt Comments for HSD17B1
HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q11-q21

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol

Molecular Function: estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity

Biological Process: steroid metabolic process; estrogen metabolic process; steroid biosynthetic process; estrogen biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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