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anti-IGF1R antibody :: Rabbit IGF1 Receptor Polyclonal Antibody

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Catalog # MBS175136
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

IGF1 Receptor (IGF1R), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Anti-IGF1 Receptor antibody

Product Synonym Names
Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; CD221 antibody; CD221 antigen antibody; IGF 1 receptor antibody; IGF 1R antibody; IGF I receptor antibody; IGF-I receptor antibody; IGF1R antibody; IGF1R_HUMAN antibody; IGFIR antibody; IGFIRC antibody; IGFR antibody; Insulin like growth factor 1 receptor antibody; Insulin like growth factor 1 receptor precursor antibody; Insulin-like growth factor 1 receptor beta chain antibody; Insulin-like growth factor I receptor antibody; JTK13 antibody; MGC142170 antibody; MGC142172 antibody; MGC18216 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 270450
3D Structure
ModBase 3D Structure for P08069
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor (1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-IGF1R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IGF1R antibody
Description: Rabbit IgG polyclonal antibody for Insulin-like growth factor 1 receptor(IGF1R) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of phosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.
Applications Tested/Suitable for anti-IGF1R antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-IGF1R antibody
Western Blot
Concentration: 0.1-0.5 ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat

Immunohistochemistry (Paraffin-embedded Section)
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested speceis with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the stianing of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-IGF1R antibody
Anti-IGF1 Receptor antibody, MBS175136, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: MCF-7 Cell Lysate

anti-IGF1R antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-IGF1R antibody
Anti-IGF1 Receptor antibody, MBS175136, IHC(P)
IHC(P): Rat Pancreas Tissue
anti-IGF1R antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for IGF1R. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
154,793 Da
NCBI Official Full Name
Insulin-like growth factor 1 receptor
NCBI Official Synonym Full Names
insulin-like growth factor 1 receptor
NCBI Official Symbol
IGF1R  [Similar Products]
NCBI Official Synonym Symbols
IGFR; CD221; IGFIR; JTK13
  [Similar Products]
NCBI Protein Information
insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
UniProt Protein Name
Insulin-like growth factor 1 receptor
UniProt Synonym Protein Names
Insulin-like growth factor I receptor; IGF-I receptor; CD_antigen: CD221Cleaved into the following 2 chains:Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain
UniProt Gene Name
IGF1R  [Similar Products]
UniProt Synonym Gene Names
IGF-I receptor  [Similar Products]
UniProt Entry Name
IGF1R_HUMAN
NCBI Summary for IGF1R
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for IGF1R
IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; identical protein binding; insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; protein-tyrosine kinase activity; insulin receptor substrate binding; phosphoinositide 3-kinase binding; G-protein alpha-subunit binding; insulin receptor binding; insulin-like growth factor receptor activity; ATP binding

Biological Process: phosphoinositide 3-kinase cascade; epidermis development; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; positive regulation of MAPKKK cascade; mammary gland development; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; axonogenesis; insulin-like growth factor receptor signaling pathway; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cell migration

Disease: Insulin-like Growth Factor I, Resistance To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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