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anti-IKK-beta antibody :: Rabbit IKK-beta Polyclonal Antibody

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Catalog # MBS9407019
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

IKK-beta, Polyclonal Antibody

Full Product Name

IKK-beta (Ab-199) Antibody

Product Synonym Names
IKK-b (Ab-199) Antibody; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK2; IkBKB; NFKBIKB
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603258
3D Structure
ModBase 3D Structure for O14920
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of total IKK-bprotein.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-b.
Target Name
IKK-b
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-IKK-beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IKK-beta antibody
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
Product Categories/Family for anti-IKK-beta antibody
Applications Tested/Suitable for anti-IKK-beta antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-IKK-beta antibody
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western Blot (WB) of anti-IKK-beta antibody
Western blot analysis of extracts from MDA cells using IKK-b(Ab-199) Antibody.
anti-IKK-beta antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-IKK-beta antibody
Immunofluorescence staining of methanol-fixed Hela cells using IKK-b(Ab-199) Antibody.
anti-IKK-beta antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for IKK-beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
79,509 Da
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
NCBI Official Synonym Full Names
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
NCBI Official Symbol
IKBKB  [Similar Products]
NCBI Official Synonym Symbols
IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
  [Similar Products]
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit beta
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
UniProt Synonym Protein Names
I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
UniProt Gene Name
IKBKB  [Similar Products]
UniProt Synonym Gene Names
IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB  [Similar Products]
UniProt Entry Name
IKKB_HUMAN
NCBI Summary for IKK-beta
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for IKK-beta
IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.10; Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; response to virus; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; B cell homeostasis; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; inflammatory response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Immunodeficiency 15
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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