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anti-IL33 antibody :: Rabbit IL-33 Polyclonal Antibody

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Catalog # MBS150331
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

IL-33 (IL33), Polyclonal Antibody

Popular Item
Also Known As

IL-33 Antibody

Product Synonym Names
IL-33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; NFHEV; Interleukin-33; Interleukin-1 family member 11; IL-33; interleukin 33
Antibody/Peptide Pairs
IL-33 peptide (MBS152579) is used for blocking the activity of IL-33 antibody (MBS150331)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
270
OMIM
608678
3D Structure
ModBase 3D Structure for O95760
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
IL-33 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the center of human IL-33.
The immunogen is located within amino acids 150 - 200 of IL-33.
Buffer
IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IL-33 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-IL33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IL33 antibody
IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
Applications Tested/Suitable for anti-IL33 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P)
Application Notes for anti-IL33 antibody
IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL.
Optimal dilutions for each application to be determined by the researcher.

POSITIVE CONTROL: 1) Cat. No. MBS151677 - A20 Cell Lysate
2) Cat. No. MBS154265 - Human Lymphoid Tissue Tissue Slide

Western Blot (WB) of anti-IL33 antibody
Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 μg/mL.
anti-IL33 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-IL33 antibody
Immunofluorescence of IL-33 in Human Lymph Node tissue with IL-33 antibody at 20 μg/mL.
anti-IL33 antibody Immunofluorescence (IF) image
Immunohistochemistry of anti-IL33 antibody
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 2.5 ug/ml
anti-IL33 antibody Immunohistochemistry (IHC) image
NCBI/Uniprot data below describe general gene information for IL33. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Predicted: 30 kDa

Observed: 36 kDa
NCBI Official Full Name
interleukin-33 isoform 1
NCBI Official Synonym Full Names
interleukin 33
NCBI Official Symbol
NCBI Official Synonym Symbols
DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
  [Similar Products]
NCBI Protein Information
interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
UniProt Protein Name
Interleukin-33
UniProt Synonym Protein Names
Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV  [Similar Products]
UniProt Entry Name
IL33_HUMAN
NCBI Summary for IL33
IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
UniProt Comments for IL33
IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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