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Rabbit Integrin b4 (Tyr-1494) Polyclonal Antibody

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Catalog # MBS472030
Unit / Price
  0.1 mL  /  $380 +1 FREE 8GB USB
Testing Data
Product Name

Integrin b4 (Tyr-1494), Polyclonal Antibody

Popular Item
Full Product Name

Integrin beta4 (Tyr-1494), phospho-specific

Product Synonym Names
Integrin b4 (Tyr-1494); phospho-specific; CD104; GP150
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Rat, Mouse
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phosphointegrin- b4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa* protein corresponding to the molecular mass of integrin-b4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.
Affinity Purified
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Phospho-integrin-b4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human integrin-b4. This peptide sequence is found in all three integrin-b4 isoforms and is highly conserved in rat and mouse integrin-b4.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Other Notes
Small volumes of anti-Integrin b4 (Tyr-1494) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Integrin b4 (Tyr-1494) antibody
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin alpha-6-beta-4receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the integrin-beta-4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly this site is critical for integrin alpha-6-beta-4 increases in carcinoma invasion.
Applications Tested/Suitable for anti-Integrin b4 (Tyr-1494) antibody
ELISA, Western Blot
ELISA: 1:4000
Western Blot: 1:2000

Product References and Citations for anti-Integrin b4 (Tyr-1494) antibody
• Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.
• Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
• Kim, H.I. et al. (2008) Cancer Prev Res. 1(5):385. (WB: Human b4 transfectants)

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