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anti-IRF-3 antibody :: Rabbit IRF-3 Polyclonal Antibody

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Catalog # MBS9502591
Unit / Price
  0.05 mg  /  $225 +1 FREE 8GB USB
  0.1 mg  /  $310 +1 FREE 8GB USB
Western Blot (WB)
Product Name

IRF-3, Polyclonal Antibody

Popular Item
Full Product Name

IRF-3 (Phospho-Ser396) Antibody

Product Synonym Names
Interferon regulatory factor 3; IRF3
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
Z56281 mRNA
3D Structure
ModBase 3D Structure for Q14653
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
IRF-3 (Phospho-Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:S396; M:S388; R:S390
Immunogen
The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser396.
Immunogen Range
362-411
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-IRF-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IRF-3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-IRF-3 antibody
WB: 1:500~1:1000
ELISA: 1:20000

Western Blot (WB) of anti-IRF-3 antibody
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser396) Antibody. The lane on the right is blocked with the phospho peptide.
anti-IRF-3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for IRF-3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47 kDa
NCBI Official Full Name
interferon regulatory factor 3 isoform 2
NCBI Official Synonym Full Names
interferon regulatory factor 3
NCBI Official Symbol
NCBI Official Synonym Symbols
IIAE7
  [Similar Products]
NCBI Protein Information
interferon regulatory factor 3
UniProt Protein Name
Interferon regulatory factor 3
UniProt Gene Name
UniProt Synonym Gene Names
IRF-3  [Similar Products]
NCBI Summary for IRF-3
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
UniProt Comments for IRF-3
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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