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anti-KCNJ1 antibody :: Goat KCNJ1/ROMK Polyclonal Antibody

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Catalog # MBS422048
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

KCNJ1/ROMK, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-KCNJ1/ROMK Antibody

Product Synonym Names
KCNJ1; potassium inwardly-rectifying channel, subfamily J, member 1; KIR1.1, ROMK, ROMK1; ATP-regulated potassium channel ROM-K; ATP-sensitive inward rectifier potassium channel 1; OTTHUMP00000045938; inwardly rectifying K+ channel; potassium inwardly-rec; KCNJ1 antibody; potassium inwardly-rectifying channel; subfamily J; member 1 antibody; KIR1.1; ROMK; ROMK1 antibody; ATP-regulated potassium channel ROM-K antibody; ATP-sensitive inward rectifier potassium channel 1 antibody; OTTHUMP00000045938 antibody; inwardly rectifying K+ channel antibody; potassium inwardly-rectifying channel J1 antibody; KCNJ1 / ROMK
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
DQININFVVD AGNEN
OMIM
241200
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DQININFVVDAGNEN, from the internal region of the protein sequence according to NP_000211.1; NP_722448.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-KCNJ1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KCNJ1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-KCNJ1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: approx. 45kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 44.8kDa according to NP_000211.1). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-KCNJ1 antibody
(1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-KCNJ1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for KCNJ1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_722448.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,611 Da
NCBI Official Full Name
ATP-sensitive inward rectifier potassium channel 1 isoform a
NCBI Official Synonym Full Names
potassium voltage-gated channel subfamily J member 1
NCBI Official Symbol
KCNJ1  [Similar Products]
NCBI Official Synonym Symbols
ROMK; ROMK1; KIR1.1
  [Similar Products]
NCBI Protein Information
ATP-sensitive inward rectifier potassium channel 1
UniProt Protein Name
ATP-sensitive inward rectifier potassium channel 1
UniProt Synonym Protein Names
ATP-regulated potassium channel ROM-K; Inward rectifier K(+) channel Kir1.1; Potassium channel, inwardly rectifying subfamily J member 1
UniProt Gene Name
KCNJ1  [Similar Products]
UniProt Synonym Gene Names
ROMK1  [Similar Products]
UniProt Entry Name
KCNJ1_HUMAN
NCBI Summary for KCNJ1
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for KCNJ1
ROMK: an inward rectifier-type potassium channel protein. Probably plays a major role in potassium homeostasis in the kidney. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. The channel is activated by internal ATP and can be blocked by external barium. High levels of expression in the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver. Three alternatively spliced isoforms have been described.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q24

Cellular Component: plasma membrane; voltage-gated potassium channel complex

Molecular Function: ATP-activated inward rectifier potassium channel activity; inward rectifier potassium channel activity; phosphatidylinositol-4,5-bisphosphate binding

Biological Process: excretion; potassium ion import; potassium ion transport

Disease: Bartter Syndrome, Antenatal, Type 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-KCNJ1 antibody
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