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anti-KDM1 antibody :: Rabbit LSD1 Polyclonal Antibody

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Catalog # MBS150364
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Western Blot (WB)
Product Name

LSD1 (KDM1), Polyclonal Antibody

Full Product Name

LSD1 Antibody

Product Synonym Names
LSD1; AOF2; KDM1; LSD1; BHC110; AOF2; KIAA0601; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; lysine (K)-specific demethylase 1
Antibody/Peptide Pairs
LSD1 peptide (MBS152798) is used for blocking the activity of LSD1 antibody (MBS150364)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
609132
3D Structure
ModBase 3D Structure for O60341
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
LSD1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
LSD1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human LSD1.
Buffer
LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
LSD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-KDM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KDM1 antibody
LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Applications Tested/Suitable for anti-KDM1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-KDM1 antibody
LSD1 antibody can be used for the detection of LSD1 by Western blot at 1 - 2 mug/mL.

Western Blot (WB) of anti-KDM1 antibody
Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 μg/mL.
anti-KDM1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for KDM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
95,155 Da
NCBI Official Full Name
lysine-specific histone demethylase 1A isoform a
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 1A
NCBI Official Symbol
KDM1A  [Similar Products]
NCBI Official Synonym Symbols
AOF2; KDM1; LSD1; BHC110
  [Similar Products]
NCBI Protein Information
lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2
UniProt Protein Name
Lysine-specific histone demethylase 1A
UniProt Synonym Protein Names
BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
UniProt Gene Name
KDM1A  [Similar Products]
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1  [Similar Products]
UniProt Entry Name
KDM1A_HUMAN
NCBI Summary for KDM1
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Comments for KDM1
LSD1: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Ubiquitously expressed. Belongs to the flavin monoamine oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Nuclear receptor co-regulator; Oxidoreductase; EC 1.-.-.-; Demethylase

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; nucleus

Molecular Function: FAD binding; p53 binding; oxidoreductase activity; transcription factor binding; histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-K4 specific); protein binding; MRF binding; enzyme binding; histone demethylase activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; chromatin binding; transcription factor activity; demethylase activity

Biological Process: negative regulation of histone H3-K9 methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; granulocyte differentiation; in utero embryonic development; positive regulation of erythrocyte differentiation; negative regulation of transcription factor activity; muscle cell development; negative regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of DNA binding; protein amino acid demethylation; regulation of transcription from RNA polymerase II promoter; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell proliferation; histone H3-K9 demethylation; pituitary gland development; positive regulation of hormone biosynthetic process; positive regulation of megakaryocyte differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of protein binding
Precautions
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Disclaimer
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