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anti-SEMA5A antibody :: Rabbit M Sema5a Polyclonal Antibody

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Catalog # MBS9206417
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

M Sema5a, Polyclonal Antibody

Popular Item
Full Product Name

M Sema5a Antibody (N-term)

Product Synonym Names
Semaphorin-5A; Semaphorin-F; Sema F; SEMA5A; SEMAF
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
609297
3D Structure
ModBase 3D Structure for Q13591
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse (Predicted Reactivity: Rat)
Specificity
This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of mouse Sema5a.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Cellular Location
Membrane; Single-pass type I membrane protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-SEMA5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SEMA5A antibody
Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.
Product Categories/Family for anti-SEMA5A antibody
Applications Tested/Suitable for anti-SEMA5A antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-SEMA5A antibody
WB~~1:1000

Western Blot (WB) of anti-SEMA5A antibody
Western blot analysis of mouse anti-Sema5a (N-term) Pab in A2058 cell line lysates (35ug/lane). Sema5a(arrow) was detected using the purified Pab.
anti-SEMA5A antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SEMA5A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
120615
NCBI Official Full Name
semaphorin-5A
NCBI Official Synonym Full Names
sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
NCBI Official Symbol
SEMA5A  [Similar Products]
NCBI Official Synonym Symbols
semF; SEMAF
  [Similar Products]
NCBI Protein Information
semaphorin-5A
UniProt Protein Name
Semaphorin-5A
UniProt Synonym Protein Names
Semaphorin-F; Sema F
Protein Family
UniProt Gene Name
SEMA5A  [Similar Products]
UniProt Synonym Gene Names
SEMAF; Sema F  [Similar Products]
UniProt Entry Name
SEM5A_HUMAN
NCBI Summary for SEMA5A
This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
UniProt Comments for SEMA5A
SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: syndecan binding; heparan sulfate proteoglycan binding; axon guidance receptor activity; semaphorin receptor binding

Biological Process: nervous system development; axon guidance; positive regulation of actin filament depolymerization; axonal fasciculation; negative regulation of cell adhesion; negative regulation of axon extension involved in axon guidance; diencephalon development; patterning of blood vessels; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; blood vessel endothelial cell proliferation during sprouting angiogenesis; cell-cell signaling; positive chemotaxis; positive regulation of axon extension involved in axon guidance; positive regulation of endothelial cell proliferation; cell adhesion
Product References and Citations for anti-SEMA5A antibody
Bialecka,M., Neurosci. Lett. 399 (1-2), 121-123 (2006)
Melin,M., Neuropsychobiology 54 (1), 64-69 (2006)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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