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anti-MIF antibody :: Goat MIF Polyclonal Antibody

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Catalog # MBS420999
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

MIF, Polyclonal Antibody

Also Known As

Goat anti-MIF Antibody

Product Synonym Names
Goat Anti-MIF, Biotinylated Antibody; MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor); HGNC: 7097; GIF; GLIF; MMIF; glycosylation-inhibiting factor; phenylpyruvate tautomerase; macrophage migration inhibitory factor; MIF antibody; macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody; HGNC: 7097 antibody; GIF antibody; GLIF antibody; MMIF antibody; glycosylation-inhibiting factor antibody; phenylpyruvate tautomerase antibody; macrophage migration inhibitory factor antibody
Product Gene Name
Matching Pairs
Unconjugated Antibody: MIF (MBS420999)
Biotin Conjugated Antibody: MIF (MBS423623)
Antibody/Peptide Pairs
MIF peptide (MBS425709) is used for blocking the activity of MIF antibody (MBS420999)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
115
Immunogen Sequence
NAANVGWNNS TFA
OMIM
153620
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-MIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MIF antibody
Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
Application Notes for anti-MIF antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration: 0.01-0.03ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml.

Western Blot (WB) of anti-MIF antibody
(0. 01ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-MIF antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-MIF antibody
(0. 01ug/ml) staining of Daudi (A) and Jurkat (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-MIF antibody Western Blot (WB) (WB) image
Testing Data of anti-MIF antibody
(1.5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).
anti-MIF antibody Testing Data image
NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,476 Da
NCBI Official Full Name
macrophage migration inhibitory factor
NCBI Official Synonym Full Names
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
NCBI Official Symbol
NCBI Official Synonym Symbols
GIF; GLIF; MMIF
  [Similar Products]
NCBI Protein Information
macrophage migration inhibitory factor
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Synonym Protein Names
Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GLIF; MMIF; MIF; GIF  [Similar Products]
UniProt Entry Name
MIF_HUMAN
NCBI Summary for MIF
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
UniProt Comments for MIF
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - tyrosine; Cytokine; Cell development/differentiation; EC 5.3.3.12; EC 5.3.2.1; Isomerase; Apoptosis; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle

Molecular Function: chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding

Biological Process: carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation

Disease: Rheumatoid Arthritis, Systemic Juvenile
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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