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anti-MLK3 antibody :: Rabbit MLK3 Polyclonal Antibody

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Catalog # MBS9386068
Unit / Price
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  0.1 mL  /  $440 +1 FREE 8GB USB
  0.2 mL  /  $715 +1 FREE 8GB USB
Western Blot (WB)
Product Name

MLK3, Polyclonal Antibody

Popular Item
Full Product Name

MLK3 (Phospho-Thr277+Ser281) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS9386068 Technical Datasheet
OMIM
U07747 mRNA
3D Structure
ModBase 3D Structure for Q16584
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
MLK3 (Phospho-Thr277+Ser281) Ab detects endogenous levels of MLK3 only when phosphorylated at Thr277+Ser281
Purity/Purification
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho-peptide affinity columns.
Concentration
1.0mg/ml (lot specific)
Subcellular location
Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent
Immunogen
A synthesized peptide derived from human MLK3 (Phospho-Thr277+Ser281)
Tissue Specifity
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
Similarity
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20°C.Stable for 12 months from date of receipt
Other Notes
Small volumes of anti-MLK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MLK3 antibody
Western Blot (WB)
Application Notes for anti-MLK3 antibody
WB 1:1000-3000
IHC 1:50-1:200

Western Blot (WB) of anti-MLK3 antibody
Western blot analysis of MLK3 (Phospho-Thr277+Ser281)using K562 whole cell lysates
anti-MLK3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MLK3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92 KD
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 11
NCBI Official Symbol
MAP3K11  [Similar Products]
NCBI Official Synonym Symbols
MLK3; PTK1; SPRK; MLK-3; MEKK11
  [Similar Products]
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 11
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 11
UniProt Synonym Protein Names
Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase
UniProt Gene Name
MAP3K11  [Similar Products]
NCBI Summary for MLK3
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]
UniProt Comments for MLK3
MLK3: a TKL kinase of the MLK family. Has an amino-terminal SH3 domain followed by the kinase domain, two leucine zipper domains, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. Phosphorylates IkappaB and activates SEK1 and MKK7.

Protein type: EC 2.7.11.25; Kinase, protein; MLK family; MLK subfamily; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: centrosome; cytoplasm; membrane; microtubule

Molecular Function: identical protein binding; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of JNK activity; activation of MAPK activity; cell proliferation; JNK cascade; microtubule-based process; positive regulation of apoptosis; positive regulation of JNK activity; positive regulation of JNK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-MLK3 antibody
 Reference Product  PubMed Publications
 MAP3K1 antibody  >1 publications with MLK3 and MAP3K1
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