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anti-MRPL53 antibody :: Rabbit MRPL53 Polyclonal Antibody

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Catalog # MBS9609430
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

MRPL53, Polyclonal Antibody

Popular Item
Full Product Name

MRPL53 Antibody

Product Synonym Names
39S ribosomal protein L53; L53mt; mitochondrial; MRP-L53; mrpl53; RM53_HUMAN
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96EL3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
MRPL53 antibody detects endogenous levels of total MRPL53
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human MRPL53
Subcellular Location
Mitochondrion.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit
Similarity
Bovine (92%), Horse (92%), Sheep (83%), Rabbit (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-MRPL53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MRPL53 antibody
Subunit Structure: Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. mL53 is located at the L7/L12 stalk.
Similarity: Belongs to the mitochondrion-specific ribosomal protein mL53 family.
Applications Tested/Suitable for anti-MRPL53 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-MRPL53 antibody
WB: 1:1000-3000
IHC: 1:200

Immunohistochemistry (IHC) of anti-MRPL53 antibody
MBS9609430 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-MRPL53 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-MRPL53 antibody
Western blot analysis of MRPL53 using HeLa whole cell lysates
anti-MRPL53 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-MRPL53 antibody
Western blot analysis of extracts from various samples, using MRPL53 Antibody. Lane 1: Mouse Myeloma cell treated with blocking peptide. Lane 2: Mouse Myeloma cell; Lane 3: Hela;
anti-MRPL53 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MRPL53. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 12 kDa
Predicted: 13 kDa
NCBI Official Full Name
39S ribosomal protein L53, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L53
NCBI Official Symbol
MRPL53  [Similar Products]
NCBI Official Synonym Symbols
L53MT
  [Similar Products]
NCBI Protein Information
39S ribosomal protein L53, mitochondrial
UniProt Protein Name
39S ribosomal protein L53, mitochondrial
UniProt Synonym Protein Names
Mitochondrial large ribosomal subunit protein mL53
Protein Family
UniProt Gene Name
MRPL53  [Similar Products]
UniProt Synonym Gene Names
L53mt; MRP-L53  [Similar Products]
NCBI Summary for MRPL53
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008]
Research Articles on MRPL53
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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