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anti-MyoD antibody :: Rabbit MyoD Polyclonal Antibody

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Catalog # MBS9402320
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Western Blot (WB)
Product Name

MyoD, Polyclonal Antibody

Also Known As

MyoD (Phospho-Ser200) Antibody

Product Synonym Names
MYF3; MYOD; MYOD1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
320
OMIM
159970
3D Structure
ModBase 3D Structure for P15172
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.
Modification
Phospho-Ser200
Target Name
MyoD
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-MyoD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MyoD antibody
MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the
Product Categories/Family for anti-MyoD antibody
Applications Tested/Suitable for anti-MyoD antibody
Western Blot (WB)
Application Notes for anti-MyoD antibody
Western blotting: 1:500~1:1000

Western Blot (WB) of anti-MyoD antibody
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD(Phospho-Ser200) Antibody.
anti-MyoD antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MyoD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,501 Da
NCBI Official Full Name
myoblast determination protein 1
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1  [Similar Products]
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
  [Similar Products]
NCBI Protein Information
myoblast determination protein 1
UniProt Protein Name
Myoblast determination protein 1
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Protein Family
UniProt Gene Name
MYOD1  [Similar Products]
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3  [Similar Products]
UniProt Entry Name
MYOD1_HUMAN
NCBI Summary for MyoD
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for MyoD
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; Transcription factor; RNA splicing

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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