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anti-MyoD antibody :: Rabbit MyoD Polyclonal Antibody

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Catalog # MBS9402320
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Western Blot (WB)
Product Name

MyoD, Polyclonal Antibody

Also Known As

MyoD (Phospho-Ser200) Antibody

Product Synonym Names
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
3D Structure
ModBase 3D Structure for P15172
Species Reactivity
Human, Mouse, Rat
The antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1.0 mg/ml (lot specific)
Immunogen Type
Immunogen Description
Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.
Target Name
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-MyoD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MyoD antibody
MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the
Product Categories/Family for anti-MyoD antibody
Applications Tested/Suitable for anti-MyoD antibody
Western Blot (WB)
Application Notes for anti-MyoD antibody
Western blotting: 1:500~1:1000

Western Blot (WB) of anti-MyoD antibody
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD(Phospho-Ser200) Antibody.
anti-MyoD antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MyoD. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,501 Da
NCBI Official Full Name
myoblast determination protein 1
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
myoblast determination protein 1
UniProt Protein Name
Myoblast determination protein 1
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Protein Family
UniProt Gene Name
MYOD1  [Similar Products]
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3  [Similar Products]
UniProt Entry Name
NCBI Summary for MyoD
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for MyoD
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; Transcription factor; RNA splicing

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
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