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anti-NEDD9 antibody :: Rabbit NEDD9 Polyclonal Antibody

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Catalog # MBS9607636
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

NEDD9, Polyclonal Antibody

Popular Item
Full Product Name

NEDD9 Antibody

Product Synonym Names
Cas like docking; Cas scaffolding protein family member 2; CAS-L; CAS2; CasL; CASL_HUMAN; CASS2; Crk associated substrate related; Crk associated substrate related protein; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ49G10.2; dJ761I2.1 (enhancer of filamentation (HEF1)); dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF 1; HEF1; NEDD-9; Nedd9; Neural precursor cell expressed developmentally down-regulated protein 9; P105; Renal carcinoma antigen NY-REN-12
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q14511
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
NEDD9 antibody detects endogenous levels of total NEDD9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human NEDD9
Subcellular Location
Cytoplasm > Cytoskeleton > Spindle and Cytoplasm > Cell cCortex. Nucleus. Golgi Apparatus. Cell projection > Lamellipodium. Cytoplasm. Cell Junction > Focal Adhesion. Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi Apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
Tissue Specificity
Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-NEDD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NEDD9 antibody
Description: The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants.
Function: Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B-and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Subunit Structure: Interacts with BCAR3 and SH2D3C (By similarity). Homodimer. Can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. Forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase. Interacts with MICAL and TXNL4/DIM1.
Post-translational Modifications: Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.PTK2/FAK1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins). The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRK and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross-linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium-and protein kinase C-dependent mechanisms and requires the integrity of the actin cytoskeleton. Phosphorylation at Ser-369 induces proteasomal degradation.
Similarity: Contains a central domain containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif confers specific interaction with the HLH proteins ID2, E12 and E47. It is absolutely required for the induction of pseudohyphal growth in yeast and mediates homodimerization and heterodimerization with p130cas.The SH3 domain interacts with two proline-rich regions of PTK2/FAK1. Belongs to the CAS family.
Applications Tested/Suitable for anti-NEDD9 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-NEDD9 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-NEDD9 antibody
MBS9607636 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-NEDD9 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-NEDD9 antibody
Western blot analysis of extracts from 293, using NEDD9 Antibody. Lane 1 was treated with the antigen-specific peptide.
anti-NEDD9 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-NEDD9 antibody
Western blot analysis of Hela whole cell lysates, using NEDD9 Antibody. The lane on the left is treated with the antigen-specific peptide.
anti-NEDD9 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-NEDD9 antibody
Western blot analysis of NEDD9 expression in Rat brain lysate
anti-NEDD9 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NEDD9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 92 kDa
Predicted: 93 kDa
NCBI Official Full Name
enhancer of filamentation 1 isoform 3
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 9
NCBI Official Symbol
NEDD9  [Similar Products]
NCBI Official Synonym Symbols
CAS2; CASL; HEF1; CAS-L; CASS2
  [Similar Products]
NCBI Protein Information
enhancer of filamentation 1
UniProt Protein Name
Enhancer of filamentation 1
UniProt Synonym Protein Names
CRK-associated substrate-related protein; CAS-L; CasL
Protein Family
UniProt Gene Name
NEDD9  [Similar Products]
UniProt Synonym Gene Names
CASL; CASS2; hEF1; CAS-L; CasL; NEDD-9  [Similar Products]
NCBI Summary for NEDD9
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Comments for NEDD9
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Pathways associated with anti-NEDD9 antibody
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