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anti-Slc12a1 antibody :: Rabbit NKCC2 Polyclonal Antibody

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Catalog # MBS805913
Unit / Price
  0.1 mg  /  $435 +1 FREE 8GB USB
Western Blot (WB)
Product Name

NKCC2 (Slc12a1), Polyclonal Antibody

Popular Item
Full Product Name

NKCC2 Antibody: HRP

Product Synonym Names
SLCl2A1 Antibody, sodium potassium chloride cotransporter 2 Antibody, sodium-potassium-chloride cotransporter isoform 2 Antibody, BSCl Antibody, MBSCl Antibody, Na-K-2CI cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody, Antibody
Matching Pairs
Unconjugated Antibody: NKCC2 (MBS805560)
HRP Conjugated Antibody: NKCC2 (MBS805913)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5PQV1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Affinity Purified
Concentration
1mg/mL (lot specific)
Conjugate
HRP
Immunogen
Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
Research Area(s)
Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
Cellular Localization
Membrane
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Certifcate of Analysis
1ug/ml of MBS805913 was sufficient for detection of NKCC2 in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Preparation and Storage
20 degree C; 1 year+. Avoid freeze/ thaw cycle.
Other Notes
Small volumes of anti-Slc12a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Slc12a1 antibody
Background Info: Detects ~160kDa

Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
Product Categories/Family for anti-Slc12a1 antibody
Applications Tested/Suitable for anti-Slc12a1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Slc12a1 antibody
WB (1:1000); optimal dilutions for assays should be determined by the user.

Western Blot (WB) of anti-Slc12a1 antibody
Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.
anti-Slc12a1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for Slc12a1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Synonym Full Names
solute carrier family 12 (sodium/potassium/chloride transporter), member 1
NCBI Official Symbol
Slc12a1  [Similar Products]
NCBI Official Synonym Symbols
BSC1; Nkcc2
  [Similar Products]
NCBI Protein Information
solute carrier family 12 member 1
UniProt Protein Name
RCG26347, isoform CRA_c
UniProt Synonym Protein Names
RCG26347, isoform CRA_c; Slc12a1 protein; Solute carrier family 12 member 1
Protein Family
UniProt Gene Name
Slc12a1  [Similar Products]
UniProt Synonym Gene Names
rCG_26347  [Similar Products]
UniProt Entry Name
Q5PQV1_RAT
NCBI Summary for Slc12a1
has bumetamide-sensitive Na(+)-(K+)-Cl- symporter activity; may play a role in sodium and chloride transport in the kidney [RGD, Feb 2006]
UniProt Comments for Slc12a1
NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Cellular Component: cell surface; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: sodium:potassium:chloride symporter activity; cation:chloride symporter activity

Biological Process: diterpenoid metabolic process; sodium ion transport; protein insertion into membrane from inner side; chloride transport; excretion; kidney development; potassium ion transport; chemical homeostasis
Product References and Citations for anti-Slc12a1 antibody
1.Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2.Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-Slc12a1 antibody
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >225 publications with Slc12a1 and Kidney
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