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anti-GRIN2B antibody :: Rabbit NMDA NR2B Subunit Polyclonal Antibody

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Catalog # MBS502087
Unit / Price
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  0.05 mL  /  $315 +1 FREE 8GB USB
Testing Data
Product Name

NMDA NR2B Subunit (GRIN2B), Polyclonal Antibody

Popular Item
Full Product Name

Anti-NMDA Receptor, NR2B Subunit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_005103.3 (233806406..234260360, complement). Location: 4q43
3D Structure
ModBase 3D Structure for Q00960
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat, mouse, human
Specificity
Specific for the ~180k NR2B subunit of the NMDA receptor. No reactivity towards the NR2A and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Purity/Purification
Neat Serum (Unpurified neat serum)
Form/Format
50 ul neat serum. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Fusion protein from the C-terminus region of the NR2B subunit of rat NMDA receptor.
Immunogen Information
Fusion protein from the C-terminal region of the NR2B subunit
Immunogen Species
Rat
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.
Biological Significance
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Preparation and Storage
Store at -20 degree C; stable for at least 1 year
Other Notes
Small volumes of anti-GRIN2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRIN2B antibody
Rabbit polyclonal antibody
Applications Tested/Suitable for anti-GRIN2B antibody
Western Blot (WB)
Application Notes for anti-GRIN2B antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-GRIN2B antibody
anti-GRIN2B antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GRIN2B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
180
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol
Grin2b  [Similar Products]
NCBI Official Synonym Symbols
GluN2B
  [Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B
UniProt Gene Name
Grin2b  [Similar Products]
UniProt Synonym Gene Names
GluN2B; NMDAR2B; NR2B  [Similar Products]
UniProt Entry Name
NMDE2_RAT
NCBI Summary for GRIN2B
subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]
UniProt Comments for GRIN2B
Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death

By similarity.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3. Interacts with HIP1 and Neto1

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with DAPK1

By similarity. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Post-translational modification: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity

By similarity.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. [View classification]
Product References and Citations for anti-GRIN2B antibody
• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.
• Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338.

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Disclaimer
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