• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-GRIN1 antibody :: Rabbit NMDAR1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS175244
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

NMDAR1 (GRIN1), Polyclonal Antibody

Popular Item
Also Known As

Anti-NMDAR1 antibody

Product Synonym Names
Glutamate[NMDA] receptor subunit zeta-1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; GluN1 antibody; Glutamate[NMDA] receptor subunit zeta 1 antibody; Glutamate[NMDA] receptor subunit zeta-1 antibody; Glutamate receptor ionotropic N methyl D aspartate 1 antibody; Grin 1 antibody; Grin1 antibody; MRD8 antibody; N methyl D aspartate receptor antibody; N methyl D aspartate receptor channel antibody; N methyl D aspartate receptor channel subunit zeta 1 antibody; N methyl D aspartate receptor subunit NR1 antibody; N-methyl-D-aspartate receptor antibody; N-methyl-D-aspartate receptor subunit NR1 antibody; NMD-R1 antibody; NMDA 1 antibody; NMDA NR1 antibody; NMDA R1 antibody; NMDA receptor 1 antibody; NMDA1 antibody; NMDAR 1 antibody; NMDAR antibody; Nmdar1 antibody; NMDZ1_HUMAN antibody; NR 1 antibody; NR1 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
938
OMIM
gene 614254
3D Structure
ModBase 3D Structure for Q05586
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, rat
Predicted to work with: Mouse
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GRIN1 (36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GRIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRIN1 antibody
Description: Rabbit IgG polyclonal antibody for Glutamate receptor ionotropic, NMDA 1(GRIN1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: The NMDA receptor(NMDAR) is a specific type of ionotropic glutamate receptor. NMDA(N-methyl D-aspartate) is the name of a selective agonist that binds to NMDA receptors but not to other glutamate receptors. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. NMDAR1 gene is mapped to 9q34.3 and encodes a 938-amino acid protein which showed high evolutionary conservation in structure and physiologic properties. It consists of 21 exons distributed over about 31 kb. Three of the exons that are alternatively spliced in the rat and which produce 8 isoforms in that species were also present in the human sequence. The promoter region contained 2 DNA binding sites for the homeobox proteins 'even-skipped'. The gene is a candidate for the site of the mutation in torsion dystonia.The NMDA receptor is a non-specific cation channel and thus directly contributes to excitatory synaptic transmission by depolarizing the postsynaptic cell. NMDA receptors are modulated by a number of endogenous and exogenous compounds and play a key role in a wide range of physiologic and pathologic processes, such as excitotoxicity.
Applications Tested/Suitable for anti-GRIN1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-GRIN1 antibody
Western Blot:
Concentration: 0.1-0.5 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: n/a

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Test Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formal/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-GRIN1 antibody
Anti-NMDAR1 antibody, MBS175244, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: HELA Cell Lysate
anti-GRIN1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GRIN1 antibody
Anti-NMDAR1 antibody, MBS175244, IHC(P)
IHC(P): Rat Brain Tissue
anti-GRIN1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for GRIN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
101,851 Da
NCBI Official Full Name
Glutamate receptor ionotropic, NMDA 1
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 1
NCBI Official Symbol
GRIN1  [Similar Products]
NCBI Official Synonym Symbols
NR1; MRD8; GluN1; NMDA1; NMDAR1
  [Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 1; NMD-R1; glutamate [NMDA] receptor subunit zeta 1; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; N-methyl-D-aspartate receptor channel, subunit zeta-1
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 1
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1
UniProt Gene Name
GRIN1  [Similar Products]
UniProt Synonym Gene Names
NMDAR1; GluN1; NMD-R1  [Similar Products]
UniProt Entry Name
NMDZ1_HUMAN
NCBI Summary for GRIN1
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for GRIN1
NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Channel, calcium

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: neuron projection; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; terminal button; excitatory synapse; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic membrane; synaptic vesicle; plasma membrane; synapse; cell junction

Molecular Function: voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; calcium channel activity; calcium ion binding; calmodulin binding; protein binding; enzyme binding; glutamate binding; extracellular-glutamate-gated ion channel activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; male mating behavior; prepulse inhibition; adult locomotory behavior; positive regulation of apoptosis; regulation of dendrite morphogenesis; rhythmic process; response to morphine; sensory perception of pain; regulation of axonogenesis; calcium ion homeostasis; synaptic transmission; regulation of respiratory gaseous exchange; conditioned taste aversion; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; protein tetramerization; cation transport; synaptic transmission, glutamatergic; response to amphetamine; social behavior; respiratory gaseous exchange; pons maturation; cellular calcium ion homeostasis; regulation of membrane potential; response to ethanol; regulation of synaptogenesis; olfactory learning; long-term memory; suckling behavior; propylene metabolic process; ionotropic glutamate receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; response to calcium ion

Disease: Mental Retardation, Autosomal Dominant 8
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-GRIN1 antibody
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000