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anti-RTN4 antibody :: Rabbit NogoA Polyclonal Antibody

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Catalog # MBS151432
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

NogoA (RTN4), Polyclonal Antibody

Full Product Name

NogoA Antibody

Product Synonym Names
NogoA; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545; KIAA0886; My043; SP1507; Reticulon-4; Foocen; Nogo protein; reticulon 4
Antibody/Peptide Pairs
NogoA peptide (MBS152822) is used for blocking the activity of NogoA antibody (MBS151432)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
604475
3D Structure
ModBase 3D Structure for Q9NQC3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.
Purity/Purification
NogoA Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
NogoA antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA.
Buffer
NogoA Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
NogoA antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-RTN4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RTN4 antibody
NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
Applications Tested/Suitable for anti-RTN4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RTN4 antibody
NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 mug/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-RTN4 antibody
Western blot analysis of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 μg/mL.
anti-RTN4 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RTN4 antibody
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 μg/mL.
anti-RTN4 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-RTN4 antibody
Immunofluorescence of NogoA in Mouse Brain cells with NogoA antibody at 20 μg/mL.
anti-RTN4 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for RTN4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
108,450 Da
NCBI Official Full Name
reticulon-4 isoform A
NCBI Official Synonym Full Names
reticulon 4
NCBI Official Symbol
NCBI Official Synonym Symbols
ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
  [Similar Products]
NCBI Protein Information
reticulon-4; foocen; Human NogoA; reticulon 5; My043 protein; neurite outgrowth inhibitor; neurite growth inhibitor 220; neuroendocrine-specific protein C homolog
UniProt Protein Name
Reticulon-4
UniProt Synonym Protein Names
Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0886; NOGO; Nogo protein; NSP  [Similar Products]
UniProt Entry Name
RTN4_HUMAN
NCBI Summary for RTN4
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for RTN4
Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Endoplasmic reticulum; Membrane protein, multi-pass; Inhibitor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cell projection; cell soma; endoplasmic reticulum; plasma membrane; integral to endoplasmic reticulum membrane; nuclear envelope; intracellular

Molecular Function: protein binding

Biological Process: regulation of apoptosis; nerve growth factor receptor signaling pathway; apoptosis; cerebral cortex radial glia guided migration; axonal fasciculation; negative regulation of axonogenesis; angiogenesis; negative regulation of axon extension; negative regulation of cell growth; regulation of axonogenesis; regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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