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anti-NR0B2 antibody :: NR0B2 Polyclonal Antibody

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Catalog # MBS127945
Unit / Price
  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Product Name

NR0B2, Polyclonal Antibody

Popular Item
Also Known As

NR0B2 Polyclonal Antibody

Product Synonym Names
SHP; SHP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
257
OMIM
phenotype 604630
3D Structure
ModBase 3D Structure for Q15466
Clonality
Polyclonal
Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Source
Rabbit
Immunogen
Recombinant protein of human NR0B2
Calculated Molecular Weight
28kDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-NR0B2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NR0B2 antibody
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
Product Categories/Family for anti-NR0B2 antibody
Applications Tested/Suitable for anti-NR0B2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NR0B2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100

Western Blot (WB) of anti-NR0B2 antibody
Western blot analysis of extracts of various cell lines, using NR0B2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
anti-NR0B2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-NR0B2 antibody
Immunohistochemistry of paraffin-embedded rat liver using NR0B2 antibody at dilution of 1:100 (40x lens).
anti-NR0B2 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-NR0B2 antibody
Immunofluorescence analysis of U2OS cells using NR0B2 antibody. Blue: DAPI for nuclear staining.
anti-NR0B2 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for NR0B2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Nuclear receptor subfamily 0 group B member 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 0, group B, member 2
NCBI Official Symbol
NR0B2  [Similar Products]
NCBI Official Synonym Symbols
SHP; SHP1
  [Similar Products]
NCBI Protein Information
nuclear receptor subfamily 0 group B member 2; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 2
UniProt Synonym Protein Names
Orphan nuclear receptor SHP; Small heterodimer partner
UniProt Gene Name
NR0B2  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
NR0B2_HUMAN
NCBI Summary for NR0B2
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]
UniProt Comments for NR0B2
NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily.

Protein type: Nuclear receptor co-regulator; Nuclear receptor; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein domain specific binding; protein binding; peroxisome proliferator activated receptor binding; protein homodimerization activity; DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription factor activity; transcription corepressor activity

Biological Process: cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; organ regeneration; negative regulation of transcription factor activity; response to glucose stimulus; positive regulation of insulin secretion; steroid hormone mediated signaling; gene expression; negative regulation of transcription from RNA polymerase II promoter

Disease: Obesity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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