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anti-NUDT21 antibody :: Rabbit NUDT21 Polyclonal Antibody

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Catalog # MBS9603395
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

NUDT21, Polyclonal Antibody

Popular Item
Full Product Name

NUDT21 Antibody

Product Synonym Names
CFIM 25; CFIM25; Cleavage and polyadenylation specific factor 5 25 kD subunit; Cleavage and polyadenylation specific factor 5 25 kDa; Cleavage and polyadenylation specific factor 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit; CPSF 25; CPSF 25 kDa subunit; CPSF 5; CPSF25; CPSF5; CPSF5_HUMAN; DKFZp686H1588; Nucleoside diphosphate linked moiety X motif 21; Nucleoside diphosphate-linked moiety X motif 21; Nudix (nucleoside diphosphate linked moiety X) type motif 21; Nudix hydrolase 21; Nudix motif 21; NUDT 21; Nudt21; Pre mRNA cleavage factor Im (25kD); Pre mRNA cleavage factor Im 25 kDa subunit; Pre mRNA cleavage factor Im 25kD subunit; Pre-mRNA cleavage factor Im 25 kDa subunit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43809
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
NUDT21 antibody detects endogenous levels of NUDT21.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human NUDT21.
Subcellular Location
Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
Tissue Specificity
Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-NUDT21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NUDT21 antibody
Function: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:15937220, PubMed:17024186, PubMed:17098938, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:17024186). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:17098938, PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:17098938, PubMed:20695905, PubMed:29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed:29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed:8626397, PubMed:14690600, PubMed:15169763, PubMed:17024186, PubMed:22813749, PubMed:20479262). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed:20479262, PubMed:21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono-and di-adenosine nucleotides (PubMed:18445629).
Subunit Structure: Homodimer (via N-and C-terminus); binds RNA as homodimer (PubMed:20695905, PubMed:18445629, PubMed:20479262). Component of the cleavage factor Im (CFIm) complex which is an heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or an heterodimer of CPSF6 and CPSF7 (PubMed:9659921, PubMed:8626397, PubMed:14561889, PubMed:20695905, PubMed:23187700, PubMed:21295486). The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery (PubMed:29276085). Interacts with CPSF6 (via the RRM domain); this interaction is direct and enhances binding to RNA (PubMed:14561889, PubMed:15169763, PubMed:17172643, PubMed:19864460, PubMed:29276085). Interacts with CPSF7 (PubMed:29276085, Ref. 29). Interacts with FIP1L1; this interaction occurs in a RNA sequence-specific manner (PubMed:15937220). Interacts with PABPN1 (PubMed:15169763). Interacts (via N-terminus) with PAPOLA (via C-terminus); this interaction is direct and diminished by acetylation (PubMed:15169763, PubMed:17172643). Interacts with SNRNP70 (PubMed:14561889).
Post-translational Modifications: Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
Similarity: Belongs to the Nudix hydrolase family. CPSF5 subfamily.
Applications Tested/Suitable for anti-NUDT21 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NUDT21 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-NUDT21 antibody
Western blot analysis of extracts from HepG2, using NUDT21 antibody. Lane 1 was treated with the blocking peptide.
anti-NUDT21 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-NUDT21 antibody
MBS9603395 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-NUDT21 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-NUDT21 antibody
Western blot analysis of extracts of HeLa cells, using NUDT21 antibody.
anti-NUDT21 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NUDT21. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 26 kDa
Predicted: 27 kDa
NCBI Official Full Name
cleavage and polyadenylation specificity factor subunit 5
NCBI Official Synonym Full Names
nudix hydrolase 21
NCBI Official Symbol
NUDT21  [Similar Products]
NCBI Official Synonym Symbols
CPSF5; CFIM25
  [Similar Products]
NCBI Protein Information
cleavage and polyadenylation specificity factor subunit 5
UniProt Protein Name
Cleavage and polyadenylation specificity factor subunit 5
UniProt Synonym Protein Names
Cleavage and polyadenylation specificity factor 25 kDa subunit; CPSF 25 kDa subunit; Cleavage factor Im complex 25 kDa subunit1 PublicationManual assertion based on opinion iniRef.1"Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits."Rueegsegger U., Blank D., Keller W.Mol. Cell 1:243-253(1998) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-73 AND 183-189, FUNCTION, IDENTIFICATION IN THE CLEAVAGE FACTOR IM COMPLEX, SUBCELLULAR LOCATION.
Protein Family
UniProt Gene Name
NUDT21  [Similar Products]
UniProt Synonym Gene Names
CPSF 25 kDa subunit; CFIm251 PublicationManual assertion based on opinion iniRef.1; Nudix motif 21  [Similar Products]
NCBI Summary for NUDT21
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008]
UniProt Comments for NUDT21
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:15937220, PubMed:17024186, PubMed:17098938, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:17024186). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:17098938, PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:17098938, PubMed:20695905, PubMed:29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed:29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed:8626397, PubMed:14690600, PubMed:15169763, PubMed:17024186, PubMed:22813749, PubMed:20479262). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed:20479262, PubMed:21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (). Binds to chromatin (). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed:18445629).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated with anti-NUDT21 antibody
 Reference Product  PubMed Publications
 CPSF6 antibody  >2 publications with NUDT21 and CPSF6
Diseases associated with anti-NUDT21 antibody
Organs/Tissues associated with anti-NUDT21 antibody
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with NUDT21 and Testis
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