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anti-NUMB antibody :: Rabbit NUMB Polyclonal Antibody

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Catalog # MBS150158
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

NUMB, Polyclonal Antibody

Full Product Name

NUMB Antibody

Product Synonym Names
NUMB; S171; C14orf41; c14_5527; Protein numb homolog; Protein S171; h-Numb; numb homolog (Drosophila)
Antibody/Peptide Pairs
NUMB peptide (MBS152195) is used for blocking the activity of NUMB antibody (MBS150158)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603728
3D Structure
ModBase 3D Structure for P49757
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
At least four isoforms of NUMB are known to exist; this antibody will detect all of them.
Purity/Purification
NUMB Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
NUMB antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human NUMB.
Buffer
NUMB Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
NUMB antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-NUMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NUMB antibody
NUMB Antibody: NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.
Applications Tested/Suitable for anti-NUMB antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NUMB antibody
NUMB antibody can be used for detection of NUMB by Western blot at 0.25 - 0.5 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-NUMB antibody
Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 μg/mL.
anti-NUMB antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-NUMB antibody
Immunofluorescence of NUMB in human lung tissue with NUMB antibody at 20 μg/mL.
anti-NUMB antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-NUMB antibody
Immunohistochemistry of NUMB in human lung tissue with NUMB antibody at 5 μg/mL.
anti-NUMB antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for NUMB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,396 Da
NCBI Official Full Name
protein numb homolog isoform 1
NCBI Official Synonym Full Names
numb homolog (Drosophila)
NCBI Official Symbol
NCBI Official Synonym Symbols
S171; C14orf41; c14_5527
  [Similar Products]
NCBI Protein Information
protein numb homolog; h-Numb
UniProt Protein Name
Protein numb homolog
UniProt Synonym Protein Names
Protein S171
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
h-Numb  [Similar Products]
UniProt Entry Name
NUMB_HUMAN
NCBI Summary for NUMB
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for NUMB
NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extrinsic to plasma membrane; focal adhesion; clathrin-coated vesicle; apical part of cell; basolateral plasma membrane; integral to plasma membrane; early endosome; plasma membrane; nucleus

Molecular Function: protein binding; cadherin binding; beta-catenin binding; alpha-catenin binding

Biological Process: positive regulation of polarized epithelial cell differentiation; axon guidance; positive regulation of neurogenesis; Notch signaling pathway; negative regulation of Notch signaling pathway; neuroblast division in the subventricular zone; lateral ventricle development; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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