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anti-USO1 antibody :: Rabbit p115 Polyclonal Antibody

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Catalog # MBS8247729
Unit / Price
  0.05 mL  /  $195 +1 FREE 8GB USB
  0.1 mL  /  $250 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

p115 (USO1), Polyclonal Antibody

Popular Item
Also Known As

Anti-p115 Antibody

Product Synonym Names
VDP; General vesicular transport factor p115; Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
973
OMIM
603344
3D Structure
ModBase 3D Structure for O60763
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of p115 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide of human p115
Preparation and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-USO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-USO1 antibody
Rabbit polyclonal antibody to p115
Applications Tested/Suitable for anti-USO1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-USO1 antibody
Western Blot: 1/500 - 1/2000
Immunohistochemistry: 1/50 - 1/200
Immunoprecipitation: 1/20 - 1/50

Western Blot (WB) of anti-USO1 antibody
Western blot analysis of p115 expression in BT474 (A), K562 (B), A549 (C) whole cell lysates.
anti-USO1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-USO1 antibody
Immunohistochemical analysis of p115 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-USO1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for USO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
general vesicular transport factor p115 isoform 1
NCBI Official Synonym Full Names
USO1 vesicle transport factor
NCBI Official Symbol
NCBI Official Synonym Symbols
TAP; VDP; P115
  [Similar Products]
NCBI Protein Information
general vesicular transport factor p115
UniProt Protein Name
General vesicular transport factor p115
UniProt Synonym Protein Names
Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein
UniProt Gene Name
UniProt Synonym Gene Names
VDP; TAP  [Similar Products]
UniProt Entry Name
USO1_HUMAN
NCBI Summary for USO1
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Comments for USO1
TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: cytosol; endoplasmic reticulum; Golgi apparatus; Golgi membrane; Golgi stack; membrane; nucleolus; transport vesicle

Molecular Function: protein binding; protein transporter activity

Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; intracellular protein transport; transcytosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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