View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit p130 Cas (Tyr-751) Polyclonal Antibody Catalog # MBS472042 Unit / Price 0.1 mL / $465 +1 FREE 8GB USB 5x0.1 mL / $1,920 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name p130 Cas (Tyr-751), Polyclonal Antibody Full Product Name Anti-p130 Cas (Tyr-751), phospho-specific Product Synonym Names p130 Cas (Tyr-751); phospho-specific; BCAR1; Breast cancer anti-estrogen resistance protein 1; CRK associated substrate; Cas Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Species Reactivity Human, Rat, Mouse Specificity This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells Purity/Purification Affinity Purified Form/Format Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Immunogen Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-657 in mouse p130 Cas. Preparation and Storage Store at -20 degree C. Do not aliquot. Stable for 1 year. Other Notes Small volumes of anti-p130 Cas (Tyr-751) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-p130 Cas (Tyr-751) antibody p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) and the FAK-related kinase PYK2, as well as with the tyrosine phosphatases PTP-1B and PTP-PEST. The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr-762 in rat or Tyr-668 in mouse p130 Cas) that can promote interaction with the SH2 domain of SFKs. The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains, including the adaptors Crk and Nck. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage. Dephosphorylation of this site occurs during apoptosis, and may facilitate p130 Cas degradation. Applications Tested/Suitable for anti-p130 Cas (Tyr-751) antibody ELISA, Western Blot Application Notes for anti-p130 Cas (Tyr-751) antibody ELISA: 1:2000Western Blot: 1:1000 Testing Data of anti-p130 Cas (Tyr-751) antibody TOP Product References and Citations for anti-p130 Cas (Tyr-751) antibody • Nakamoto, T. et al. (1996) J Biol Chem. 271 (15):8959.• Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.• Kim, D.H. et al. (2003) Biochem Biophys Res Comm. 300:141.• Shin, N.-Y. et al. (2004) J Biol Chem. 279 (37):38331. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP