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anti-PAPSS1 antibody :: Rabbit PAPSS1 Polyclonal Antibody

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Catalog # MBS9133793
Unit / Price
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  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Western Blot (WB)
Product Name

PAPSS1, Polyclonal Antibody

Full Product Name

PAPSS1 Polyclonal Antibody

Product Synonym Names
ATPSK1; PAPSS; SK1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
SEYEKPEAPE LVLKTDSCDV NDCVQQVVEL LQERDIVPVD ASYEVKELYV PENKLHLAKT DAETLPALKI N
OMIM
Y10387 mRNA
3D Structure
ModBase 3D Structure for O43252
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human PAPSS1
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Positive Samples
LO2, HT-29, 293T, Mouse brain, Rat kideny
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-PAPSS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PAPSS1 antibody
Applications Tested/Suitable for anti-PAPSS1 antibody
Western Blot (WB)
Application Notes for anti-PAPSS1 antibody
WB: 1:500 - 1:2000

Western Blot (WB) of anti-PAPSS1 antibody
Western blot analysis of extracts of various cell lines, using PAPSS1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
anti-PAPSS1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PAPSS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Calculated: 70kDa
Observed: 71kDa
NCBI Official Full Name
bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
NCBI Official Synonym Full Names
3'-phosphoadenosine 5'-phosphosulfate synthase 1
NCBI Official Symbol
PAPSS1  [Similar Products]
NCBI Official Synonym Symbols
SK1; PAPSS; ATPSK1
  [Similar Products]
NCBI Protein Information
bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
UniProt Protein Name
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
UniProt Synonym Protein Names
3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase
UniProt Gene Name
PAPSS1  [Similar Products]
UniProt Synonym Gene Names
ATPSK1; PAPSS; SAT  [Similar Products]
NCBI Summary for PAPSS1
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]
UniProt Comments for PAPSS1
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:9576487, PubMed:9668121, PubMed:9648242, PubMed:14747722). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (PubMed:9576487).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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