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anti-PCNA antibody :: Rabbit PCNA Polyclonal Antibody

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Catalog # MBS125201
Unit / Price
  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Product Name

PCNA, Polyclonal Antibody

Popular Item
Full Product Name

PCNA Polyclonal Antibody

Product Synonym Names
PCNA; MGC8367
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 615919
3D Structure
ModBase 3D Structure for P12004
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PCNA
Calculated Molecular Weight
29/31kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-PCNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PCNA antibody
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
Product Categories/Family for anti-PCNA antibody
Applications Tested/Suitable for anti-PCNA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-PCNA antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
IP: 1:20 - 1:50

Western Blot (WB) of anti-PCNA antibody
Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
anti-PCNA antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded human colon using PCNA antibody at dilution of 1:200 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded human gastric using PCNA antibody at dilution of 1:200 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded mouse lung using PCNA antibody at dilution of 1:200 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody at dilution of 1:100 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody at dilution of 1:100 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody at dilution of 1:100 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded mouse liver using PCNA antibody at dilution of 1:100 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PCNA antibody
Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody at dilution of 1:100 (40x lens).
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PCNA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
261
NCBI Official Full Name
Proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
NCBI Official Synonym Symbols
ATLD2
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NCBI Protein Information
proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Synonym Protein Names
Cyclin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
PCNA_HUMAN
NCBI Summary for PCNA
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
UniProt Comments for PCNA
PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding

Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; response to lipid; DNA repair; leading strand elongation; G1/S-specific transcription in mitotic cell cycle; telomere maintenance via semi-conservative replication; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle

Disease: Ataxia-telangiectasia-like Disorder 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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