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anti-PIWIL1 antibody :: Rabbit PIWI-L1 Polyclonal Antibody

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Catalog # MBS150841
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

PIWI-L1 (PIWIL1), Polyclonal Antibody

Full Product Name

PIWI-L1 Antibody

Product Synonym Names
PIWI-L1; HIWI; MIWI; PIWI; CT80.1; HIWI; Piwi-like protein 1; piwi-like 1 (Drosophila)
Antibody/Peptide Pairs
PIWI-L1 peptide (MBS152665) is used for blocking the activity of PIWI-L1 antibody (MBS150841)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
605571
3D Structure
ModBase 3D Structure for Q96J94
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
PIWI-L1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.
Buffer
PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PIWI-L1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-PIWIL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PIWIL1 antibody
PIWI-L1 Antibody: PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.
Applications Tested/Suitable for anti-PIWIL1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PIWIL1 antibody
PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PIWIL1 antibody
Western blot analysis of PIWI-L1 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
anti-PIWIL1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-PIWIL1 antibody
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 μg/mL.
anti-PIWIL1 antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-PIWIL1 antibody
Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 μg/mL.
anti-PIWIL1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PIWIL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
89,484 Da
NCBI Official Full Name
PIWI protein
NCBI Official Synonym Full Names
piwi-like RNA-mediated gene silencing 1
NCBI Official Symbol
PIWIL1  [Similar Products]
NCBI Official Synonym Symbols
HIWI; MIWI; PIWI; CT80.1
  [Similar Products]
NCBI Protein Information
piwi-like protein 1; piwi homolog
UniProt Protein Name
Piwi-like protein 1
Protein Family
UniProt Gene Name
PIWIL1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PIWL1_HUMAN
NCBI Summary for PIWIL1
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Comments for PIWIL1
PIWIL1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: polysome; mRNA cap complex; cytoplasm

Molecular Function: mRNA binding; protein binding; single-stranded RNA binding; protein kinase binding

Biological Process: regulation of translation; meiotic cell cycle; multicellular organismal development; gene expression; RNA-mediated gene silencing; spermatid development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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