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anti-POU2AF1 antibody :: Rabbit POU2AF1 Polyclonal Antibody

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Catalog # MBS9127086 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

POU2AF1, Polyclonal Antibody

Popular Item
Full Product Name

POU2AF1 Polyclonal Antibody

Product Synonym Names
BOB1; OBF1; OCAB; OBF-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601206
3D Structure
ModBase 3D Structure for Q16633
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human POU2AF1
Calculated Molecular Weight
27kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-POU2AF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-POU2AF1 antibody
Applications Tested/Suitable for anti-POU2AF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-POU2AF1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-POU2AF1 antibody
Western blot analysis of extracts of various cell lines, using POU2AF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
anti-POU2AF1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-POU2AF1 antibody
Immunofluorescence analysis of HeLa cells using POU2AF1 antibody. Blue: DAPI for nuclear staining.
anti-POU2AF1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for POU2AF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
256
NCBI Official Full Name
POU domain class 2-associating factor 1
NCBI Official Synonym Full Names
POU class 2 associating factor 1
NCBI Official Symbol
POU2AF1  [Similar Products]
NCBI Official Synonym Symbols
BOB1; OBF1; OCAB; OBF-1
  [Similar Products]
NCBI Protein Information
POU domain class 2-associating factor 1; BOB-1; OCA-B; OCT-binding factor 1; B-cell-specific coactivator OBF-1; POU domain, class 2, associating factor 1
UniProt Protein Name
POU domain class 2-associating factor 1
UniProt Synonym Protein Names
B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1
UniProt Gene Name
POU2AF1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
OBF1_HUMAN
UniProt Comments for POU2AF1
POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.

Protein type: Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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