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anti-PP2A antibody :: Rabbit PP2A Polyclonal Antibody

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Catalog # MBS551072
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
  0.2 mg  /  $495 +1 FREE 8GB USB
Immunohistochemistry
Product Name

PP2A, Polyclonal Antibody

Full Product Name

Rabbit anti PP2A Polyclonal

Product Synonym Names
Rabbit anti PP2A Polyclonal purified; PP2A; PPP2CA polyclonal
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
176915
3D Structure
ModBase 3D Structure for P67775
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Serum IgG fraction was purified by Protein G affinity chromatography.
Form/Format
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Source Note
Polyclonal Protein Phosphatase 2A-PP2A antibody was produced from sera of mice immunized with synthetic protein containingPP2A antigenic determinants.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Other Notes
Small volumes of anti-PP2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PP2A antibody
Applications Tested/Suitable for anti-PP2A antibody
Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
Application Notes for anti-PP2A antibody
ELISA: Use at a concentration range of 1-2ug/ml.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of PPFE rat hippocampus tissue expressing PP2A.

Immunohistochemistry of anti-PP2A antibody
anti-PP2A antibody Immunohistochemistry (IHC) image
NCBI/Uniprot data below describe general gene information for PP2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,594 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Synonym Protein Names
Replication protein C
Protein Family
UniProt Gene Name
PPP2CA  [Similar Products]
UniProt Synonym Gene Names
PP2A-alpha; RP-C  [Similar Products]
UniProt Entry Name
PP2AA_HUMAN
NCBI Summary for PP2A
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
UniProt Comments for PP2A
PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein dimerization activity; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of growth; regulation of cell differentiation; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; regulation of cell adhesion; second-messenger-mediated signaling; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; regulation of protein catabolic process; gene expression; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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