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anti-PPP1R12A antibody :: Rabbit PPP1R12A Polyclonal Antibody

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Catalog # MBS2529823
Unit / Price
  0.02 mL  /  $110 +1 FREE 8GB USB
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Product Name

PPP1R12A, Polyclonal Antibody

Popular Item
Also Known As

PPP1R12A Polyclonal Antibody

Product Synonym Names
MBS; M130; MYPT1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1030
OMIM
602021
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen affinity purification
Concentration
1.3mg/mL (lot specific)
Immunogen
Recombinant protein of human PPP1R12A
Calculated Molecular Weight: 115kDa
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PPP1R12A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PPP1R12A antibody
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC.
Applications Tested/Suitable for anti-PPP1R12A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PPP1R12A antibody
WB: 1:1000-1:5000
IHC: 1:25-1:100

Western Blot (WB) of anti-PPP1R12A antibody
Western Blot analysis of NIH/3T3, A172, hela and PC3 cell using PPP1R12A Polyclonal Antibody at dilution of 1:350
anti-PPP1R12A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PPP1R12A antibody
Immunohistochemistry of paraffin-embedded Human thyroid cancer using PPP1R12A Polyclonal Antibody at dilution of 1:25
anti-PPP1R12A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PPP1R12A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
105,641 Da
NCBI Official Full Name
PPP1R12A protein
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 12A
NCBI Official Symbol
PPP1R12A  [Similar Products]
NCBI Official Synonym Symbols
MBS; M130; MYPT1
  [Similar Products]
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12A; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12A
UniProt Synonym Protein Names
Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit
UniProt Gene Name
PPP1R12A  [Similar Products]
UniProt Synonym Gene Names
MBS; MYPT1; Myosin phosphatase target subunit 1  [Similar Products]
UniProt Entry Name
MYPT1_HUMAN
NCBI Summary for PPP1R12A
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
UniProt Comments for PPP1R12A
MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q15-q21

Cellular Component: nucleoplasm; kinetochore; centrosome; focal adhesion; contractile fiber; cytoplasm; actin cytoskeleton

Molecular Function: enzyme inhibitor activity; protein binding; signal transducer activity; phosphatase regulator activity; protein kinase binding

Biological Process: regulation of cell adhesion; mitosis; centrosome organization and biogenesis; regulation of nucleocytoplasmic transport; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; G2/M transition of mitotic cell cycle; signal transduction; protein amino acid dephosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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