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anti-PRDM16 antibody :: Rabbit PRDM16 Polyclonal Antibody

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Catalog # MBS150905
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

PRDM16, Polyclonal Antibody

Popular Item
Also Known As

PRDM16 Antibody

Product Synonym Names
PRDM16; MEL1; LVNC8; PFM13; CMD1LL; KIAA1675; MEL1; PR domain zinc finger protein 16; PR domain-containing protein 16; PR domain containing 16
Antibody/Peptide Pairs
PRDM16 peptide (MBS152900) is used for blocking the activity of PRDM16 antibody (MBS150905)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1,092
OMIM
gene 615373
3D Structure
ModBase 3D Structure for Q9HAZ2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
PRDM16 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16.
Buffer
PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PRDM16 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-PRDM16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PRDM16 antibody
PRDM16 Antibody: PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
Applications Tested/Suitable for anti-PRDM16 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PRDM16 antibody
PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PRDM16 antibody
Western blot analysis of PRDM16 in rat brain tissue lysate with PRDM16 antibody at (A) 1 and (B) 2 μg/mL.
anti-PRDM16 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-PRDM16 antibody
Immunofluorescence of PRDM16 in Human Brain cells with PRDM16 antibody at 20 μg/mL.
anti-PRDM16 antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-PRDM16 antibody
Immunohistochemistry of PRDM16 in rat brain tissue with PRDM16 antibody at 2.5 μg/mL.
anti-PRDM16 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PRDM16. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
120,338 Da
NCBI Official Synonym Full Names
PR domain containing 16
NCBI Official Symbol
PRDM16  [Similar Products]
NCBI Official Synonym Symbols
MEL1; LVNC8; PFM13; CMD1LL
  [Similar Products]
NCBI Protein Information
PR domain zinc finger protein 16
UniProt Protein Name
PR domain zinc finger protein 16
UniProt Synonym Protein Names
PR domain-containing protein 16; Transcription factor MEL1; MDS1/EVI1-like gene 1
UniProt Gene Name
PRDM16  [Similar Products]
UniProt Synonym Gene Names
KIAA1675; MEL1; PFM13; MDS1/EVI1-like gene 1  [Similar Products]
UniProt Entry Name
PRD16_HUMAN
NCBI Summary for PRDM16
The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Comments for PRDM16
PRDM16: binds DNA and functions as a transcriptional regulator. A repressor of TGF-beta signaling. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. Four human isoforms are produced by alternative promoter usage and alternative splicing

Protein type: Methyltransferase, protein lysine, predicted; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1p36.23-p33

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: protein binding; transcription activator binding; sequence-specific DNA binding; metal ion binding; transcription coactivator activity; SMAD binding

Biological Process: regulation of cellular respiration; tongue development; neurogenesis; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; white fat cell differentiation; brown fat cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of granulocyte differentiation

Disease: Left Ventricular Noncompaction 8
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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