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anti-PRICKLE1 antibody :: Rabbit PRICKLE1 Polyclonal Antibody

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Catalog # MBS150079
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

PRICKLE1, Polyclonal Antibody

Full Product Name

PRICKLE1 Antibody

Product Synonym Names
PRICKLE1; RILP; EPM1B; RILP; Prickle-like protein 1; prickle homolog 1
Antibody/Peptide Pairs
PRICKLE1 peptide (MBS152296) is used for blocking the activity of PRICKLE1 antibody (MBS150079)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 612437
3D Structure
ModBase 3D Structure for Q96MT3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
Purity/Purification
PRICKLE1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1.
Buffer
PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PRICKLE1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-PRICKLE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PRICKLE1 antibody
PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.
Applications Tested/Suitable for anti-PRICKLE1 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PRICKLE1 antibody
PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-PRICKLE1 antibody
Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 μg/mL.
anti-PRICKLE1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-PRICKLE1 antibody
Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 μg/mL.
anti-PRICKLE1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for PRICKLE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
94,300 Da
NCBI Official Full Name
prickle-like protein 1
NCBI Official Synonym Full Names
prickle homolog 1 (Drosophila)
NCBI Official Symbol
PRICKLE1  [Similar Products]
NCBI Official Synonym Symbols
RILP; EPM1B
  [Similar Products]
NCBI Protein Information
prickle-like protein 1; prickle-like 1; REST/NRSF-interacting LIM domain protein 1; REST (RE-1 silencing transcription factor)/NRSF (neuron-restrictive silencer factor)-interacting LIM domain protein
UniProt Protein Name
Prickle-like protein 1
UniProt Synonym Protein Names
REST/NRSF-interacting LIM domain protein 1
Protein Family
UniProt Gene Name
PRICKLE1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PRIC1_HUMAN
NCBI Summary for PRICKLE1
This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]
UniProt Comments for PRICKLE1
PRICKLE1: Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure. Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor. Defects in PRICKLE1 are the cause of progressive myoclonic epilepsy type 1B (EPM1B). EPM1B is an autosomal recessive disorder characterized by myoclonus that progresses in severity over time, tonic-clonic seizures and ataxia. Defects in PRICKLE1 may be a cause of susceptibility to neural tube defects (NTD). Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components. Belongs to the prickle / espinas / testin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: nuclear membrane; cytosol; nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: positive regulation of protein ubiquitination; protein import into nucleus; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; neural tube closure; negative regulation of transcription, DNA-dependent

Disease: Epilepsy, Progressive Myoclonic 1b
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-PRICKLE1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with PRICKLE1 and Brain
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