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anti-PRLR antibody :: Rabbit Prolactin Receptor Polyclonal Antibody

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Catalog # MBS176232
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

Prolactin Receptor (PRLR), Polyclonal Antibody

Popular Item
Also Known As

Anti-Prolactin Receptor antibody

Product Synonym Names
Prolactin receptor; prolactin receptor; AI987712 antibody; CLONE SPM213 antibody; CPRLP antibody; Delta 4-delta 7/11 truncated prolactin receptor antibody; Delta 4-SF1b truncated prolactin receptor antibody; hPRL receptor antibody; hPRLrI antibody; Lactogen receptor antibody; MGC105486 antibody; OPR antibody; OTTHUMP00000115998 antibody; Pr-1 antibody; Pr-3 antibody; PRL R antibody; PRL-R antibody; PRLR antibody; Prlr-rs1 antibody; PRLR_HUMAN antibody; Prolactin receptor a antibody; Prolactin receptor antibody; Prolactin receptor delta 7/11 antibody; RATPRLR antibody; Secreted prolactin binding protein antibody; Truncated testis-specific box 1-C prolactin receptor antibody; wu:fj65c07 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
608
3D Structure
ModBase 3D Structure for Q08501
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, Mouse
Predicted to work with: Rat
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Prolactin Receptor (591-605aa QLGRLDYLDPTCFMH), identical to the related rat sequence, and different from the related human sequence by three amino acids.
Gene Name
Prolactin receptor
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PRLR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PRLR antibody
Description: Rabbit IgG polyclonal antibody for Prolactin receptor(PRLR) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: PRLR(Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR(conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.
Applications Tested/Suitable for anti-PRLR antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PRLR antibody
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-PRLR antibody
Figure 1. Western blot analysis of PRLR using anti- PRLR antibody (MBS176232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PRLR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.
anti-PRLR antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PRLR antibody
Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Human Mammary Cancer Tissue
anti-PRLR antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PRLR antibody
Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Rat Testis Tissue
anti-PRLR antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PRLR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,633 Da
NCBI Official Full Name
Prolactin receptor
NCBI Official Synonym Full Names
prolactin receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
Pr-1; Pr-3; AI987712; Prlr-rs1
  [Similar Products]
NCBI Protein Information
prolactin receptor; PRL-R; prolactin receptor related sequence 1
UniProt Protein Name
Prolactin receptor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PRL-R  [Similar Products]
UniProt Entry Name
PRLR_MOUSE
UniProt Comments for PRLR
PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; intracellular

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; metal ion binding; peptide hormone binding; protein kinase binding; prolactin receptor activity

Biological Process: lactation; regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of epithelial cell differentiation; JAK-STAT cascade
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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