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anti-POMC antibody :: Goat Proopiomelanocortin/POMC Polyclonal Antibody

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Catalog # MBS420379
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

Proopiomelanocortin/POMC, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-Proopiomelanocortin/POMC Antibody

Product Synonym Names
POMC; proopiomelanocortin; Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating; hormone/ beta-endorphin; LPH; NPP; OTTHUMP00000119991; OTTHUMP00000200964; adrenocortic; POMC antibody; proopiomelanocortin antibody; Precursor of MSH; POC; ACTH; CLIP; adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin antibody; LPH antibody; NPP antibody; OTTHUMP00000119991 antibody; OTTHUMP00000200964 antibody; adrenocorticotropic hormone antibody; adrenocorticotropin antibody; alpha-melanocyte-stimulating hormone antibody; beta-LPH antibody; beta-MSH antibody; beta-melanocyte-stimulating hormone antibody; corticotropin-like intermediary peptide antibody; gamma-LPH antibody; gamma-MSH antibody; lipotropin beta antibody; lipotropin gamma antibody; melanotropin alpha antibody; melanotropin beta antibody; melanotropin gamma antibody; met-enkephalin antibody; pro-ACTH-endorphin antibody; proopiomelanocortin preproprotein antibody; Proopiomelanocortin; Proopiomelanocortin / POMC
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS420379 Technical Datasheet
Immunogen Sequence
NAIIKNAYKK GE
OMIM
176830
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-NAIIKNAYKKGE, from the C Terminus of the protein sequence according to NP_000930.1; NP_001030333.1.
Epitope
C Terminus
Note
Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-POMC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-POMC antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-POMC antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 29kDa band observed in lysates of cell line NIH3T3 (calculated MW of 29.4kDa according to NP_000930.1 and NP_001030333.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-POMC antibody
(0.1ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-POMC antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-POMC antibody
(2ug/ml) staining of paraffin embedded Human Pituitary Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
anti-POMC antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for POMC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001030333.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,424 Da
NCBI Official Full Name
pro-opiomelanocortin preproprotein
NCBI Official Synonym Full Names
proopiomelanocortin
NCBI Official Symbol
NCBI Official Synonym Symbols
LPH; MSH; NPP; POC; ACTH; CLIP
  [Similar Products]
NCBI Protein Information
pro-opiomelanocortin
UniProt Protein Name
Pro-opiomelanocortin
UniProt Synonym Protein Names
Corticotropin-lipotropin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
POMC; ACTH; CLIP  [Similar Products]
UniProt Entry Name
COLI_HUMAN
NCBI Summary for POMC
This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jan 2016]
UniProt Comments for POMC
POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; extracellular region; extracellular space; peroxisomal matrix; peroxisome; secretory granule

Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding; type 3 melanocortin receptor binding; type 4 melanocortin receptor binding

Biological Process: cell-cell signaling; cellular pigmentation; generation of precursor metabolites and energy; glucose homeostasis; negative regulation of tumor necrosis factor production; neuropeptide signaling pathway; peptide hormone processing; positive regulation of transcription from RNA polymerase II promoter; regulation of appetite; regulation of blood pressure; signal transduction

Disease: Obesity; Proopiomelanocortin Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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