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anti-SPRY4 antibody :: Rabbit Prouty 4 Polyclonal Antibody

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Catalog # MBS9608118
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Prouty 4 (SPRY4), Polyclonal Antibody

Popular Item
Also Known As

Prouty 4 Antibody

Product Synonym Names
Protein sprouty homolog 4; Sprouty homolog 4; sprouty homolog 4 (Drosophila); SPRY 4; Spry-4; Spry4; SPY4_HUMAN
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
299
3D Structure
ModBase 3D Structure for Q9C004
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
prouty 4 antibody detects endogenous levels of total prouty 4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human prouty 4
Subcellular Location
Cytoplasm. Cell Projection > Ruffle Membrane.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-SPRY4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SPRY4 antibody
Function: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.
Subunit Structure: Interacts with the C-terminus of TESK1. Interacts with RAF1.
Similarity: The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. Belongs to the sprouty family.
Applications Tested/Suitable for anti-SPRY4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-SPRY4 antibody
WB: 1:1000-3000
IHC: 1:200

Immunohistochemistry (IHC) of anti-SPRY4 antibody
MBS9608118 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-SPRY4 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-SPRY4 antibody
Western blot analysis of prouty 4 using K562 whole cell lysates
anti-SPRY4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SPRY4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 33 kDa
Predicted: 33 kDa
NCBI Official Full Name
protein sprouty homolog 4 isoform 2
NCBI Official Synonym Full Names
sprouty RTK signaling antagonist 4
NCBI Official Symbol
SPRY4  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
protein sprouty homolog 4
UniProt Protein Name
Protein sprouty homolog 4
Protein Family
UniProt Gene Name
SPRY4  [Similar Products]
UniProt Synonym Gene Names
Spry-4  [Similar Products]
NCBI Summary for SPRY4
This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Comments for SPRY4
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-SPRY4 antibody
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