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anti-RAC1 antibody :: Rabbit RAC1 Polyclonal Antibody

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Catalog # MBS9127955
Unit / Price
  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Product Name

RAC1, Polyclonal Antibody

Popular Item
Also Known As

RAC1 Polyclonal Antibody

Product Synonym Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1; ras-related C3 botulinum toxin substrate 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
211
OMIM
602048
3D Structure
ModBase 3D Structure for P63000
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC1 (NP_008839.2).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-RAC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RAC1 antibody
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Applications Tested/Suitable for anti-RAC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresence (IF)
Application Notes for anti-RAC1 antibody
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Western Blot (WB) of anti-RAC1 antibody
Western blot analysis of extracts of various cell lines, using RAC1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
anti-RAC1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RAC1 antibody
Immunohistochemistry of paraffin-embedded human gastric using RAC1 antibody at dilution of 1:100 (40x lens).
anti-RAC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-RAC1 antibody
Immunofluorescence analysis of A549 cells using RAC1 antibody. Blue: DAPI for nuclear staining.
anti-RAC1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for RAC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
ras-related C3 botulinum toxin substrate 1 isoform Rac1
NCBI Official Synonym Full Names
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
NCBI Official Symbol
NCBI Official Synonym Symbols
MIG5; Rac-1; TC-25; p21-Rac1
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NCBI Protein Information
ras-related C3 botulinum toxin substrate 1
UniProt Protein Name
Ras-related C3 botulinum toxin substrate 1
UniProt Synonym Protein Names
Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
RAC1_HUMAN
NCBI Summary for RAC1
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for RAC1
RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: extrinsic to plasma membrane; Golgi membrane; focal adhesion; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; actin filament; trans-Golgi network; cytosol; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; thioesterase binding; Rho GDP-dissociation inhibitor binding; protein kinase binding; Rab GTPase binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; metabolic process; cell motility involved in cell locomotion; regulation of cell migration; small GTPase mediated signal transduction; positive regulation of stress fiber formation; cell adhesion; bone resorption; platelet activation; anatomical structure morphogenesis; mast cell chemotaxis; dendrite morphogenesis; positive regulation of Rho protein signal transduction; regulation of defense response to virus by virus; negative regulation of interleukin-23 production; T cell costimulation; cerebral cortex radially oriented cell migration; actin cytoskeleton organization and biogenesis; axon guidance; cell-matrix adhesion; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; ephrin receptor signaling pathway; response to wounding; inflammatory response; regulation of hydrogen peroxide metabolic process; lamellipodium biogenesis; intercellular junction assembly and maintenance; embryonic olfactory bulb interneuron precursor migration; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of phosphoinositide 3-kinase activity; engulfment of apoptotic cell; G-protein coupled receptor protein signaling pathway; cell proliferation; hyperosmotic response; positive regulation of actin filament polymerization; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; negative regulation of receptor-mediated endocytosis; positive regulation of protein amino acid phosphorylation; cell motility; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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