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anti-REST / NRSF antibody :: Rabbit REST / NRSF Polyclonal Antibody

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Catalog # MBS544698
Unit / Price
  0.1 mg Biotin  /  $495 +1 FREE 8GB USB
anti-REST / NRSF antibody
Product Name

REST / NRSF, Polyclonal Antibody

Also Known As

REST / NRSF Antibody BIOTIN-Conjugated

Product Synonym Names
Neural Restrictive Silencer Factor; NRSF; RE1 silencing transcription factor; Repressor binding to the X2 box; REST; X2 box repressor; XBR antibody
Matching Pairs
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1097
OMIM
600571
3D Structure
ModBase 3D Structure for Q13127
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
BIOTIN-Conjugated
Concentration
0.52-0.54 ug/ul in antibody stabilization buffer (lot specific)
Immunogen
Synthetic peptide taken within amino acid region 1000-1097 on human REST protein.
Expression
Ubiquitous, expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovaries.
Molecular Function
Repressor, Chromatin binding, Core promoter binding
Structure
Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL.
Subcellular Location
Nucleus
Preparation and Storage
-20 degree C for long term storage
Other Notes
Small volumes of anti-REST / NRSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-REST / NRSF antibody
BIOTIN-Conjugated RE1-silencing transcription factor Antibody
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes.
Applications Tested/Suitable for anti-REST / NRSF antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-REST / NRSF antibody
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
NCBI/Uniprot data below describe general gene information for REST / NRSF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
119,083 Da
NCBI Official Full Name
RE1-silencing transcription factor
NCBI Official Synonym Full Names
RE1 silencing transcription factor
NCBI Official Symbol
NCBI Official Synonym Symbols
WT6; XBR; NRSF
  [Similar Products]
NCBI Protein Information
RE1-silencing transcription factor
UniProt Protein Name
RE1-silencing transcription factor
UniProt Synonym Protein Names
Neural-restrictive silencer factor; X2 box repressor
UniProt Gene Name
UniProt Synonym Gene Names
NRSF; XBR  [Similar Products]
UniProt Entry Name
REST_HUMAN
NCBI Summary for REST / NRSF
This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
UniProt Comments for REST / NRSF
REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: chromatin; cytosol; nucleoplasm; nucleus; transcriptional repressor complex

Molecular Function: chromatin binding; histone deacetylase activity; metal ion binding; outward rectifier potassium channel activity; protein binding; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: hemopoietic progenitor cell differentiation; negative regulation of aldosterone biosynthetic process; negative regulation of calcium ion-dependent exocytosis; negative regulation of cell proliferation; negative regulation of insulin secretion; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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