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anti-RNASEH2A antibody :: Rabbit RNAse H2A Polyclonal Antibody

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Catalog # MBS151275
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

RNAse H2A (RNASEH2A), Polyclonal Antibody

Full Product Name

RNAse H2A Antibody

Product Synonym Names
RNAse H2A; AGS4; JUNB; RNHL; RNHIA; RNASEHI; Ribonuclease H2 subunit A; Aicardi-Goutieres syndrome 4 protein; RNase H2 subunit A; ribonuclease H2, subunit A
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 610333
3D Structure
ModBase 3D Structure for O75792
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
RNAse H2A Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A.
Buffer
RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
RNAse H2A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-RNASEH2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RNASEH2A antibody
RNAse H2A Antibody: Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.
Applications Tested/Suitable for anti-RNASEH2A antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-RNASEH2A antibody
RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 4 mug/mL.

Western Blot (WB) of anti-RNASEH2A antibody
Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 μg/mL.
anti-RNASEH2A antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-RNASEH2A antibody
Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 μg/mL.
anti-RNASEH2A antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-RNASEH2A antibody
Immunofluorescence of Rnse H2A in Hela cells with Rnse H2A antibody at 4.75 μg/mL.
anti-RNASEH2A antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for RNASEH2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,395 Da
NCBI Official Full Name
Ribonuclease H2 subunit A
NCBI Official Synonym Full Names
ribonuclease H2, subunit A
NCBI Official Symbol
RNASEH2A  [Similar Products]
NCBI Official Synonym Symbols
AGS4; JUNB; RNHL; RNHIA; RNASEHI
  [Similar Products]
NCBI Protein Information
ribonuclease H2 subunit A; RNase H(35); RNase H2 subunit A; RNase HI large subunit; ribonuclease HI subunit A; ribonuclease HI large subunit; ribonuclease H2, large subunit; ribonuclease HI, large subunit; aicardi-Goutieres syndrome 4 protein
UniProt Protein Name
Ribonuclease H2 subunit A
UniProt Synonym Protein Names
Aicardi-Goutieres syndrome 4 protein; AGS4; RNase H(35); Ribonuclease HI large subunit; RNase HI large subunit; Ribonuclease HI subunit A
Protein Family
UniProt Gene Name
RNASEH2A  [Similar Products]
UniProt Synonym Gene Names
RNASEHI; RNHIA; RNase H2 subunit A; AGS4; RNase HI large subunit  [Similar Products]
UniProt Entry Name
RNH2A_HUMAN
NCBI Summary for RNASEH2A
The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]
UniProt Comments for RNASEH2A
RNASEH2A: Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase HII family. Eukaryotic subfamily.

Protein type: Ribonuclease; EC 3.1.26.4; DNA replication

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleus

Molecular Function: RNA binding; metal ion binding; ribonuclease H activity; ribonuclease activity

Biological Process: mismatch repair; RNA catabolic process; DNA replication

Disease: Aicardi-goutieres Syndrome 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-RNASEH2A antibody
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