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anti-SIK2 antibody :: Rabbit SIK2 Polyclonal Antibody

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Catalog # MBS150970
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

SIK2, Polyclonal Antibody

Full Product Name

SIK2 Antibody

Product Synonym Names
SIK2; QIK; SNF1LK2; LOH11CR1I; KIAA0781; QIK; Qin-induced kinase; SIK-2; salt-inducible kinase 2
Antibody/Peptide Pairs
SIK2 peptide (MBS153082) is used for blocking the activity of SIK2 antibody (MBS150970)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
608973
3D Structure
ModBase 3D Structure for Q9H0K1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
SIK2 antibody is predicted to not cross-react with other SIK protein family members.
Purity/Purification
SIK2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
SIK2 antibody was raised against a 16 amino acid synthetic peptide near the center of human SIK2.
Buffer
SIK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SIK2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-SIK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SIK2 antibody
SIK2 Antibody: Salt-inducible kinase 2 (SIK2), like its closely related homolog SIK1, belongs AMPK subfamily of the Ser/Thr protein kinase family and negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1 alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.
Applications Tested/Suitable for anti-SIK2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-SIK2 antibody
SIK2 antibody can be used for detection of SIK2 by Western blot at 1 mug/mL.

Western Blot (WB) of anti-SIK2 antibody
Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.
anti-SIK2 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-SIK2 antibody
Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.
anti-SIK2 antibody Immunofluorescence (IF) image
Immunocytochemistry (ICC) of anti-SIK2 antibody
Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.
anti-SIK2 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for SIK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
103,915 Da
NCBI Official Full Name
serine/threonine-protein kinase SIK2
NCBI Official Synonym Full Names
salt-inducible kinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
QIK; SIK-2; SNF1LK2; LOH11CR1I
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase SIK2; SNF1-like kinase 2; qin-induced kinase; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine-protein kinase SNF1-like kinase 2
UniProt Protein Name
Serine/threonine-protein kinase SIK2
UniProt Synonym Protein Names
Qin-induced kinase; Salt-inducible kinase 2; SIK-2; Serine/threonine-protein kinase SNF1-like kinase 2
UniProt Gene Name
UniProt Synonym Gene Names
; SIK-2  [Similar Products]
UniProt Entry Name
SIK2_HUMAN
UniProt Comments for SIK2
QIK: a serine/threonine kinase of the CAMKL family and related to AMPK. Specifically expressed in adipose tissues. Like AMPK, QIK is phosphorylated and activated by LKB1. Part of a molecular complex including TORC2 and calcineurin that regulates the effects of circulating glucose and gut hormones during feeding on TORC2-mediated gene expression. In mice, insulin promotes the phosphorylation and ubiquitin-dependent degradation of TORC2, inhibiting gluconeogenic gene expression during re-feeding. In response to insulin, Akt2 phosphorylates and activates QIK which in turn stimulates the phosphorylation and cytoplasmic translocation of TORC2 where it associates with COP1, which promotes its ubiquitination and proteasomal degradation. Phosphorylates Ser794 of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: regulation of insulin receptor signaling pathway; protein amino acid autophosphorylation; insulin receptor signaling pathway; protein amino acid phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-SIK2 antibody
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