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anti-Sin3B antibody :: Rabbit Sin3B Polyclonal Antibody

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Catalog # MBS9602242
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Sin3B, Polyclonal Antibody

Popular Item
Full Product Name

Sin3B Antibody

Product Synonym Names
Histone deacetylase complex subunit Sin3b; Paired amphipathic helix protein Sin3b; SIN3 homolog B transcription regulator; SIN3 transcription regulator family member B; SIN3 transcription regulator homolog B; Transcriptional corepressor Sin3b
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O75182
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Sin3B antibody detects endogenous levels of total Sin3B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Sin3B
Subcellular Location
Nuclear
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-Sin3B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Sin3B antibody
Function: Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.
Subunit Structure: Interacts with FOXK1/MNF, MXI, MAD, NCOR1 and SAP30. Interaction with SUDS3 enhances the interaction with HDAC1 to form a complex. Interacts with CRY1, HCFC1, MAD3, MAD4, MAEL, REST, RNF220 and SETDB1. Interacts with C6orf89 (PubMed:23460338). Interacts with MYT1L (By similarity).
Post-translational Modifications: Ubiquitinated by RNF220 that leads to proteasomal degradation.
Applications Tested/Suitable for anti-Sin3B antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-Sin3B antibody
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-Sin3B antibody
MBS9602242 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-Sin3B antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-Sin3B antibody
Western blot analysis Sin3B using A549 whole cell lysates
anti-Sin3B antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-Sin3B antibody
MBS9602242 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-Sin3B antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for Sin3B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 130 kDa
Predicted: 134 kDa
NCBI Official Full Name
paired amphipathic helix protein Sin3b isoform 2
NCBI Official Synonym Full Names
SIN3 transcription regulator family member B
NCBI Official Symbol
SIN3B  [Similar Products]
NCBI Protein Information
paired amphipathic helix protein Sin3b
UniProt Protein Name
Paired amphipathic helix protein Sin3b
UniProt Synonym Protein Names
Histone deacetylase complex subunit Sin3b; Transcriptional corepressor Sin3b
UniProt Gene Name
SIN3B  [Similar Products]
UniProt Comments for Sin3B
Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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