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anti-SKAP2 antibody :: Rabbit SKAP2 Polyclonal Antibody

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Catalog # MBS9603313
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

SKAP2, Polyclonal Antibody

Popular Item
Full Product Name

SKAP2 Antibody

Product Synonym Names
Fyn associated phosphoprotein SKAP55 homologue; MGC10411; MGC33; PRAP; Pyk2/RAFTK associated protein; Pyk2/RAFTK-associated protein; RA70; Retinoic acid-induced protein 70; SAPS; SKAP-55HOM; SKAP-HOM; skap2; SKAP2_HUMAN; SKAP55 homolog; SKAP55R; Src associated adaptor protein; Src family associated phosphoprotein 2; Src family-associated phosphoprotein 2; src kinase associated phosphoprotein of 55 related protein; Src kinase-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domains
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O75563
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
SKAP2 antibody detects endogenous levels of SKAP2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human SKAP2.
Subcellular Location
Cytoplasm.
Tissue Specificity
Ubiquitously expressed. Present in platelets (at protein level).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-SKAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SKAP2 antibody
Function: May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.
Subunit Structure: Homodimer (By similarity). Interacts with PTPNS1. Part of a complex consisting of SKAP2, FYB1 and PTPNS1. Part of a complex consisting of SKAP2, FYB1 and LILRB3. May interact with actin (By similarity). Interacts with FYB1, which is required for SKAP2 protein stability. Interacts with LAT, GRB2, PTK2B and PRAM1. May interact with FYN, HCK and LYN. Interacts with FASLG.
Post-translational Modifications: Phosphorylated in resting platelets. Phosphorylated by FYN on Tyr-261 upon T-cell activation (Probable). Dephosphorylated on Tyr-75 by PTPN22.
Similarity: The SH3 domain interacts with FYB1 and PTK2B. Belongs to the SKAP family.
Applications Tested/Suitable for anti-SKAP2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SKAP2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-SKAP2 antibody
Western blot analysis of extracts from mouse spleen, using SKAP2 antibody. Lane 1 was treated with the blocking peptide.
anti-SKAP2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SKAP2 antibody
MBS9603313 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-SKAP2 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-SKAP2 antibody
Western blot analysis of extracts of human liver tissue, using SKAP2 antibody.
anti-SKAP2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SKAP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 55 kDa
Predicted: 42 kDa
NCBI Official Full Name
src kinase-associated phosphoprotein 2 isoform 2
NCBI Official Synonym Full Names
src kinase associated phosphoprotein 2
NCBI Official Symbol
SKAP2  [Similar Products]
NCBI Official Synonym Symbols
PRAP; RA70; SAPS; SCAP2; SKAP55R; SKAP-HOM
  [Similar Products]
NCBI Protein Information
src kinase-associated phosphoprotein 2
UniProt Protein Name
Src kinase-associated phosphoprotein 2
UniProt Synonym Protein Names
Pyk2/RAFTK-associated protein; Retinoic acid-induced protein 70; SKAP55 homolog; SKAP-55HOM; SKAP-HOM; Src family-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domains
UniProt Gene Name
SKAP2  [Similar Products]
UniProt Synonym Gene Names
PRAP; RA70; SAPS; SCAP2; SKAP55R; SKAP-55HOM; SKAP-HOM  [Similar Products]
NCBI Summary for SKAP2
The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
UniProt Comments for SKAP2
May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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